Synthesis and characterization of zinc oxide nanoparticles from Morus nigra and its anticancer activity of AGS gastric cancer cells
Synthesis and characterization of zinc oxide nanoparticles from Morus nigra and its anticancer activity of AGS gastric cancer cells
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桑树氧化锌纳米粒子的合成、表征及其对AGS胃癌细胞的抗癌活性
DOI：
10.1016/j.jphotobiol.2019.111698
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发表时间：
2020-01-01
影响因子：
5.4
通讯作者：
Wei, Xiaowei
中科院分区：
文献类型：
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作者：
Tang, Qing;Xia, Haiyan;Wei, Xiaowei
在全球范围内，胃癌是最主要的常规肿瘤。即使是成功。强化治疗方法和早期发现并不能降低胃癌的死亡率和发病率范围。本研究计划开发Mows黑质氧化锌纳米颗粒（MN-ZnONPs），并对其进行紫外可见光谱、TEM、SEM、FT-IR、EDX和XRD等不同的表征方法的评价。此外，通过细胞活力、AO/EtBr诱导的凋亡形态学变化、线粒体膜电位（MMP）的改变、细胞周期阻滞、脂质过氧化状态（TBARS）、抗氧化剂（SOD、GSH和CAT）和ROS的产生，分析MN-ZnONPs对AGS细胞的抗癌作用。利用RT-PCR技术分析凋亡基因Bax、caspase-9、caspase-3、Bcl-2的表达情况。我们观察到合成的MN-ZnONPs具有球形、结晶性和不同的官能团。我们还观察到MN-ZnONPs处理胃癌细胞表现出细胞死亡。MN-ZnONPs通过增强ROS的形成、降低MMP、凋亡形态学改变来诱导细胞凋亡，AO/EtBr观察到脂质过氧化增加、抗氧化剂减少和诱导细胞周期阻滞。进一步观察MN-ZnONPs通过改变细胞凋亡标志物的基因表达来诱导细胞凋亡的分子机制。
Gastric cancer was a foremost one among the majority of regular carcinoma cases globally. Even the achieve. ments on enhanced treatment approaches and early findings cannot decrease the mortality and morbidity ranges of gastric cancer. This current work was planned to develop Mows nigra-loaded zinc oxide nanoparticles (MN-ZnONPs) and to evaluate the different characteristic methods likes UV-vis spectroscopy, TEM, SEM, FT-IR, EDX and XRD. Furthermore, the anticancer effect of MN-ZnONPs against AGS cells were analysed via cell viability, apoptotic morphological variations by AO/EtBr, alterations of mitochondrial membrane potential (MMP), cell cycle arrest, lipid peroxidation status (TBARS), antioxidants (SOD, GSH and CAT) and generation of ROS. Moreover, the status of apoptosis gene such as Bax, caspase-9, caspase-3 and Bcl-2 expressions was analysed by using RT-PCR techniques. We observed the synthesized MN-ZnONPs have a spherical shape, crystalline nature and present different functional groups. We also observed that gastric cancer cells demonstrated in cell death by MN-ZnONPs treatments. The MN-ZnONPs induced apoptosis by enhanced formation of ROS, decreased MMP, apoptotic morphological modifications were evaluated by AO/EtBr, increased lipid peroxidation, decreased antioxidants and induced cell cycle arrest were observed. Furthermore, to confirm the molecular mechanism demonstrated of MN-ZnONPs to induce apoptosis by altering the gene expressions of apoptosis markers were observed.