CHANGES IN G0 STATE OF WI-38 FIBROBLASTS AT DIFFERENT TIMES AFTER CONFLUENCE
CHANGES IN G0 STATE OF WI-38 FIBROBLASTS AT DIFFERENT TIMES AFTER CONFLUENCE
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DOI：
10.1016/0014-4827(74)90789-7
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发表时间：
1974-01-01
影响因子：
3.7
通讯作者：
BASERGA, R
中科院分区：
文献类型：
--
作者：
AUGENLICHT, LH;BASERGA, R
发现受刺激增殖的 WI-38 人二倍体成纤维细胞在复制前阶段的一些事件是细胞静止时间长度的函数。铺板后 5 天，当细胞首次汇合时，营养变化刺激下的复制前阶段相对较短，刺激后 8 小时开始 DNA 合成，并且染色质模板活性没有增加。铺板后 9 天，受刺激细胞的预复制期延长至 14 小时，并且在刺激后 1 小时内染色质模板活性增加。最后，在 18 天的细胞中，复制前阶段甚至进一步延长至 20 小时，并且在刺激后染色质模板活性增加之前存在一个滞后。据推测，汇合的 WI-38 细胞最初在 G 1 中停滞，随后进入 G 0，并在保持静止状态时继续深入到 G 0。
Some events in the prereplicative phase of WI-38 human diploid fibroblasts stimulated to proliferate are found to be a function of the length of time the cells have been quiescent. At 5 days after plating, when the cells first become confluent, the prereplicative phase upon stimulation by a nutritional change is relatively short, DNA synthesis begins at 8 h after stimulation, and there is no increase in chromatin template activity. At 9 days after plating the prereplicative phase of stimulated cells is lengthened to 14 h and there is an increase in chromatin template activity within 1 h of stimulation. Finally, in 18-day cells, the prereplicative phase is lengthened even further to 20 h, and there is a lag after stimulation before the increase in chromatin template activity. It is proposed that confluent WI-38 cells initially arrest in G 1, subsequently pass into G 0, and continue to go deeper into G 0 as they remain quiescent.