Molecular analyses of the intestinal microbiota of chimpanzees in the wild and in captivity
Molecular analyses of the intestinal microbiota of chimpanzees in the wild and in captivity
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DOI：
10.1002/ajp.20351
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发表时间：
2007-04-01
影响因子：
2.4
通讯作者：
Ushida, Kazunari
中科院分区：
文献类型：
--
作者：
Uenishi, Gentaro;Fujita, Shiho;Ushida, Kazunari
由于野外研究肠道细菌的技术困难，有关野生黑猩猩肠道细菌的信息很少。在这项研究中，进行基于分子的细菌分析，以克服这一困难，因为聚合酶链反应（PCR）为基础的方法，如温度梯度凝胶电泳（TGGE）和扩增核糖体DNA限制性分析（ARMIA）的细菌16 S rRNA基因，可以应用于乙醇固定的粪便样品。常见的细菌属于梭菌属。rRNA亚组XIVa，如粗瘤胃球菌和真杆菌属，是由阿雷基什指出的博苏野生黑猩猩。TGGE对部分16 S rDNA的分层聚类分析表明，野生和圈养黑猩猩之间的肠道菌群组成的系统性差异。然而，几个TGGE条带通常共享野生和捕获黑猩猩被切除，并获得它们的序列。它们可能是纤细梭菌亚群细菌、类加氏乳杆菌和假小链双歧杆菌-或B。链状细菌这些可能被认为是黑猩猩常见的肠道细菌，并可能在几代人之间垂直传播。
Little information is available regarding the intestinal bacteria of chimpanzees in the wild, due to the technical difficulties of studying intestinal bacteria in the field. In this study, molecular-based bacterial analyses were performed to overcome this difficulty because polymerase chain reaction (PCR)-based methods, such as temperature gradient gel electrophoresis (TGGE) and amplified ribosomal DNA restriction analysis (ARDRA), of the bacterial 16S rRNA gene can be applied to ethanol-fixed fecal samples. The common presence of bacteria belonging to the Clostridium. rRNA sub-group XIVa, such as Ruminococcus obeum and Eubacterium sp., was indicated for Bossou wild chimpanzees by ARDRA. TGGE on partial 16S rDNA followed by hierarchical clustering analysis showed a systematic difference in the composition of intestinal microbiota between wild and captive chimpanzees. However, several TGGE bands commonly shared by wild and captured chimpanzees were excised, and their sequences were obtained. They were suggested to be the Clostridium leptum subgroup bacteria, Lactobacillus gasseri-like bacterium, and Bifidobacterium pseudocatenulatum- or B. catenulatum-like bacterium. These may be considered as common intestinal bacteria for chimpanzees, and may be transmitted vertically over generations.