Gene expression profiles of the muscarinic acetylcholine receptors in brain regions relating to filial imprinting of newly-hatched domestic chicks
Gene expression profiles of the muscarinic acetylcholine receptors in brain regions relating to filial imprinting of newly-hatched domestic chicks
复制标题
DOI：
10.1016/j.bbr.2021.113708
复制
发表时间：
2021-12-13
影响因子：
2.7
通讯作者：
Homma, Koichi J.
中科院分区：
文献类型：
--
作者：
Mori, Chihiro;Aoki, Naoya;Homma, Koichi J.
中枢神经系统中的毒蕈碱型乙酰胆碱受体（mAChR）在调节复杂功能如学习、记忆和选择性注意中起重要作用。已经在哺乳动物中鉴定了mAChR的五种亚型（M-1-M-5），并且将其分为两个亚家族：兴奋性（M-1、M-3和M-5）和抑制性（M-2和M-4）亚家族。家鸡的亲子印记是研究早期学习记忆形成机制的一个有用的实验模型。我们最近发现，在中间内侧中皮层（IMM）的mAChRs参与的印记记忆的形成。然而，每个mAChR亚型在包括IMM在内的大脑区域中的表达谱仍然未被探索。在这里，我们展示了参与印记的套膜区域中每种mAChR亚型的独特基因表达。在兴奋性mAChRs方面，M-5在IMM区和腭的其他部分表达，而M-3在IMM中表达较少，但在上腭和巢皮质中表达较高。关于抑制性mAChRs，M-2稀疏分布，但在整个苍白球区域的一些细胞中明显。M-4在腭板的IMM区和其他部位高度表达。这些表达谱可以作为基础，了解胆碱能调制的记忆形成的印记和其他学习过程中的鸟类，并与哺乳动物相比。
Muscarinic acetylcholine receptors (mAChRs) in the central nervous system play an important role in regulating complex functions such as learning, memory, and selective attention. Five subtypes of the mAChRs (M-1-M-5) have been identified in mammals, and are classified into two subfamilies: excitatory (M-1, M-3, and M-5) and inhibitory (M-2 and M-4) subfamilies. Filial imprinting of domestic chicks is a useful model in the laboratory to investigate the mechanisms of memory formation in early learning. We recently found that mAChRs in the intermediate medial mesopallium (IMM) are involved in the memory formation of imprinting. However, expression profiles of each mAChR subtype in the brain regions including the IMM remain unexplored. Here we show the unique gene expression of each mAChR subtype in the pallial regions involved in imprinting. In terms of the excitatory mAChRs, M-5 was expressed in the IMM region and other parts of the pallium, whereas M-3 was less expressed in the IMM but highly expressed in the hyperpallium and nidopallium. Regarding the inhibitory mAChRs, M-2 was sparsely distributed but clearly in some cells throughout the pallial regions. M-4 was highly expressed in the IMM region and other parts of the pallium. These expression profiles can be used as a basis for understanding cholinergic modulation in the memory formation of imprinting and other learning processes in birds, and compared to those of mammals.