INHIBITION OF THE PRAUSNITZ-KUSTNER REACTION BY AN IMMUNOGLOBULIN EPSILON-CHAIN FRAGMENT SYNTHESIZED IN ESCHERICHIA-COLI
INHIBITION OF THE PRAUSNITZ-KUSTNER REACTION BY AN IMMUNOGLOBULIN EPSILON-CHAIN FRAGMENT SYNTHESIZED IN ESCHERICHIA-COLI
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DOI：
10.1038/315577a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
GOULD, H
中科院分区：
文献类型：
--
作者：
GEHA, RS;HELM, B;GOULD, H
Prausnitz-Küstner (P-K) 反应是一种敏感测试，用于检测皮肤中免疫球蛋白 E 的存在和活性，免疫球蛋白 E 是介导过敏反应的一类重要的免疫球蛋白。此处评估了编码 IgE 恒定区 (Cε2-4) 第二、第三和第四结构域的人骨髓瘤 NDε 链基因片段抑制体内 P-K 反应的能力。将片段注射到健康成人的皮肤部位可防止随后含有针对豚草抗原的 IgE 抗体的血清的致敏。抑制 P-K 反应需要 Cε 片段比致敏血清中 IgE 摩尔过量 200 倍。 Cε片段抑制P-K反应的功效在阻断浓度和阻断效果持续时间方面均优于天然骨髓瘤IgE（PS）。 Cε2-4 片段对 P-K 反应的抑制是特异性的，可能是由于重组基因产物使肥大细胞 1,2 上的 IgE 受体饱和所致。
The Prausnitz–Küstner (P–K) reaction is a sensitive test for the presence and activity in the skin of immunoglobulin E, an important class of immunoglobulin mediating allergic reactions. A fragment of the human myeloma NDε-chain gene, encoding the second, third and fourth domains of the IgE constant region (Cε2–4) was assessed here for its ability to inhibit the P–K reactionin vivo. Injection of the fragment in skin sites of healthy human adults prevented subsequent sensitization with serum containing IgE antibody to ragweed antigen. Inhibition of the P–K reaction required a 200-fold molar excess of the Cεfragment over the IgE present in the sensitizing serum. The efficacy of the Cεfragment in inhibiting the P–K reaction compared favourably with that of natural myeloma IgE (PS) in terms of both blocking concentrations and duration of the blocking effect. The inhibition of the P–K reaction by Cε2–4 fragments was specific and probably caused by the saturation of IgE receptors on mast cells1,2by the recombinant gene product.