Evidence for nonadrenergic inhibitory nerves in the guinea pig trachealis muscle.
Evidence for nonadrenergic inhibitory nerves in the guinea pig trachealis muscle.
复制标题
豚鼠气管肌中非肾上腺素能抑制神经的证据。
DOI：
10.1152/ajplegacy.1973.224.5.1072
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
T. Tomita
中科院分区：
文献类型：
--
作者：
R. Coburn;T. Tomita
科伯恩，r.f.和t.富田。豚鼠气管肌存在非肾上腺素能抑制性神经的证据。点。[j] .中国生物医学工程学报，2016,31(5):344 - 344。1973年的今天，我们研究了豚鼠气管肌的抑制性神经支配，方法是将肌条置于含有97 y. 02和3 To . CO2平衡的克雷布斯溶液的器官浴中，并在海莨菪碱或阿托品存在的情况下测量电场刺激对等长张力的影响。a)心得安（l-6 × 10B6 g/ml）或普萘洛尔（l-5 × 10B4 g/ml）对电场刺激引起的松弛无显著影响。b)约4 mg/kg利血平预处理后，动物出现大面积刺激松弛。这些条带与去甲肾上腺素孵育不影响松弛的大小。利血平后未见儿茶酚胺荧光。c)肌肉对溴化钠（1 -6 × 1 Om6 g/ml）的肾上腺素能神经元阻断作用不敏感。d)与其他组织的交感神经刺激相比，在较低的刺激频率下引起最大松弛。这些发现表明，所有或大部分抑制性神经支配是非肾上腺素能性的。颈气管肌含有一些肾上腺素能神经，这可以通过β -阻断药物局部抑制场刺激松弛和对溴胺肾上腺素能神经元阻断作用的敏感性来证明。
COBURN, R. F., AND T. TOMITA. Evidence for nonadrenergic inhibitory nerves in the guinea pig trachealis muscle. Am. J. Physiol. 224(5) : 1072-1080. 1973-The inhibitory innervation of the guinea pig trachealis muscle was studied by placing muscle strips in an organ bath containing Krebs solution equilibrated with 97 y. 02 and 3 To CO2 and measuring the effects of field stimulation on isometric tension in the presence of hyoscine or atropine. In strips obtained from the thoracic trachea: a) relaxations elicited by field stimulation were not sigificantly influenced by propranolol (l-6 X 10B6 g/ml) or practalol (l-5 X 10B4 g/ml). b) Large fieldstimulation relaxations were seen after pretreatment of the animals with approximately 4 mg/kg reserpine. Incubation of these strips with norepinephrine did not influence the magnitude of the relaxations. Catecholamine fluorescence could not be seen after reserpine. c) The muscle was not sensitive to the adrenergic neuron blocking effects of bretylium ( l-6 X 1 Om6 g/ml). d) Maximal relaxations were elicited at lower stimulus frequencies than found with sympathetic nerve stimulation in other tissues. These findings suggest that all or most of the inhibitory innervation is nonadrenergic. Cervical trachealis muscle contained some adrenergic nerves as evidenced by partial suppression of field-stimulation relaxations with beta-blocking drugs and sensitivity to the adrenergic neuron-blocking effects of bretylium.