Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo
Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo
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DOI：
10.1359/jbmr.1997.12.12.2040
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发表时间：
1997-12-01
影响因子：
6.2
通讯作者：
MacDougall, M
中科院分区：
文献类型：
--
作者：
DSouza, RN;Cavender, A;MacDougall, M
尽管前牙本质转化为牙本质的确切机制尚不清楚，但一些证据表明非胶原蛋白（NCP）是牙本质生物矿化的重要调节剂。在这里，我们比较了两个牙本质NCP基因、牙本质基质蛋白1（Dmp1）和牙本质唾液酸磷蛋白（DSPP）在发育中磨牙中的体内时空表达模式，使用Dmp1和DSPP特异性引物组在胚胎第13天至1天大的第一磨牙上进行逆转录聚合酶链反应，Dmp1转录物出现在芽期后期，而DSPP mRNA出现在帽期，这两个基因的表达在整个牙形成过程中持续存在，原位杂交分析揭示了这些基因的表达模式存在有趣的差异，虽然Dmp1和DSPP在矿化开始前在年轻的成牙本质细胞中表现出共表达，但这些基因的表达在后期显着不同，矿物质出现后分泌性成牙本质细胞中Dmp1表达下降，而成牙本质细胞中仍维持高水平的DSPP。在早期分泌性成釉细胞中，DSPP 表达是短暂的，并且随着牙本质基质的出现而下调。有趣的是，在成釉细胞成熟 Dbase 期间，Dmp1 表达在成釉细胞中变得明显。与牙齿特异性的 Dspp 表达相反，Dmp1 在骨骼骨化过程中由成骨细胞表达，针对 DSPP 基因的“DSP”和“DPP”区域的探针显示出相同的 mRNA 表达模式。这些数据表明，Dmp1 和 DSPP 的发育表达模式是不同的，这意味着这些分子在体内发挥不同的生物学功能。
Although the precise mechanisms of the conversion of predentin to dentin are not well understood, several lines of evidence implicate the noncollagenous proteins (NCPs) as important regulators of dentin biomineralization. Here we compared the in vivo temporospatial expression patterns of two dentin NCP genes, dentin matrix protein 1 (Dmp1), and dentin sialophosphoprotein (DSPP) in developing molars, Reverse transcription-polymerase chain reaction was performed on embryonic day 13 to 1-day-old first molars using Dmp1- and DSPP-specific primer sets, Dmp1 transcripts appeared at the late bud stage, while DSPP mRNA was seen at the cap stage, Expression of both genes was sustained throughout odontogenesis, In situ hybridization analysis revealed interesting differences in the expression patterns of these genes, While Dmp1 and DSPP showed coexpression fn young odontoblasts before the start of mineralization, the expression of these genes was notably distinct at later stages, Dmp1 expression decreased in secretory odontoblasts after the appearance of mineral, while high levels of DSPP were sustained hi odontoblasts. In early secretory ameloblasts, DSPP expression was transient and down-regulated with the appearance of dentin matrix, Interestingly, Dmp1 expression became evident in ameloblasts during the maturative Dbase of amelogenesis. In contrast to Dspp expression that was tooth-specific, Dmp1 was expressed by osteoblasts throughout ossification in the skeleton, Probes directed to the ''DSP'' and ''DPP'' regions of the DSPP gene showed identical patterns of mRNA expression, These data show that the developmental expression patterns of Dmp1 and DSPP are distinct, implying that these molecules serve different biological functions in vivo.