1α,25-Dihydroxyvitamin D3 inhibits CD40L-induced pro-inflammatory and immunomodulatory activity in Human Monocytes
1α,25-Dihydroxyvitamin D3 inhibits CD40L-induced pro-inflammatory and immunomodulatory activity in Human Monocytes
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DOI：
10.1016/j.cyto.2008.12.009
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发表时间：
2009-03-01
期刊：
影响因子：
3.8
通讯作者：
Bergamini, Alberto
中科院分区：
文献类型：
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作者：
Almerighi, Cristiana;Sinistro, Anna;Bergamini, Alberto
CD40配体(CD40L)刺激单核细胞具有促炎症和免疫调节活性。在这里，我们报道了类固醇激素1α，25-二羟基维生素D3(1,25133)对CD40L配体刺激的人血单核细胞的影响。CD40L刺激的单核细胞与1，25D3共同处理后，肿瘤坏死因子α和白介素1β的产生和分泌减少，表面共刺激分子CD80和CD86的表达减少。此外，CD40L和1，25D3共同处理的单核细胞对CD4+T细胞的共刺激作用导致细胞增殖减少，并降低了干扰素-γ，但增加了IL-10的产生。最后，1，25D3干扰了CD40L对去血清诱导的单核细胞凋亡的保护作用。这些发现表明，1，25D3可能调节单核细胞与表达CD40L的T细胞或其他细胞类型的相互作用，从而影响免疫或炎症反应的结果。(C)2008爱思唯尔有限公司。保留所有权利。
CD40 ligand (CD40L) stimulation induces proinflammatory and immunomodulatory activity in monocytes. Here, we report on the effects of the steroid hormone 1 alpha,25-dihydroxyvitamin D3 (1,25133) on human blood monocytes that have been stimulated with the CD40L ligand. Co-treatment of CD40L-stimulated monocytes with 1,25D3 resulted in reduced production and secretion of tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta, as well as in reduced expression of the surface co-stimulatory molecules CD80 and CD86. In addition, costimulation of CD4+ T lymphocytes by monocytes co-treated with CD40L and 1,25D3 resulted in reduced cell proliferation and diminished interferon (IFN)-gamma but enhanced IL-10 production by CD4+ T cells. Finally, 1,25D3 interfered with the ability of CD40L to rescue monocytes from apoptosis induced by serum withdrawal. These findings suggest that 1,25D3 may regulate the interaction of monocytes with T cells or other cell types that express CD40L, thus influencing the outcome of the immune or inflammatory response. (C) 2008 Elsevier Ltd. All rights reserved.