Altered expression of goblet cell‐ and mucin glycosylation‐related genes in the intestinal epithelium during infection with the nematode Nippostrongylus brasiliensis in rat
Altered expression of goblet cell‐ and mucin glycosylation‐related genes in the intestinal epithelium during infection with the nematode Nippostrongylus brasiliensis in rat
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DOI：
10.1111/j.1600-0463.2006.apm_353.x
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发表时间：
2006-04
期刊：
影响因子：
2.8
通讯作者：
J. Yamauchi;Y. Kawai;M. Yamada;R. Uchikawa;T. Tegoshi;N. Arizono
中科院分区：
文献类型：
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作者：
J. Yamauchi;Y. Kawai;M. Yamada;R. Uchikawa;T. Tegoshi;N. Arizono
肠道线虫感染可诱导明显的杯状细胞增生和粘液分泌，但其调控机制仍有待阐明。在本研究中，在巴西日本圆线虫感染后的不同时间从大鼠小肠分离上皮细胞，并通过半定量逆转录（RT）-PCR估计杯状细胞和粘蛋白糖基化相关基因的表达水平。在研究的基因中，粘蛋白核心肽（MUC）2，唾液酸转移酶（Siat）4c和三叶因子家族（TFF）3早在感染后2 - 4天就上调，这表明它们与早期先天保护性反应有关。感染后7天及之后，当线虫达到成熟时，观察到MUC3、MUC4、类粘蛋白分子β（Relm β）和3O-磺基转移酶（3ST）1的显著上调，而在大多数蠕虫从肠道排出后，3ST 2表达水平增加。在肥大细胞缺陷型Ws/Ws大鼠中也观察到糖基化相关基因表达的类似改变，表明上皮中的肥大细胞与这些基因的上调无关。目前的发现是，在感染的时间过程中，每个杯状细胞或糖基化相关基因的表达水平发生了不同的变化，这表明感染后粘液层发生了连续的质变。
Intestinal nematode infection induces marked goblet cell hyperplasia and mucus secretion, but the mechanisms of regulation of the changes still remain to be elucidated. In the present study, epithelial cells were isolated from the rat small intestine at various times after Nippostrongylus brasiliensis infection, and the levels of expression of goblet cell‐ and mucin glycosylation‐related genes were estimated by semi‐quantitative reverse transcription (RT)‐PCR. Among the genes investigated, mucin core peptide (MUC) 2, sialyltransferase (Siat) 4c and trefoil factor family (TFF) 3 were upregulated as early as 2–4 days post‐infection, suggesting that they are associated with an early innate protective response. Seven days post‐infection and thereafter, when the nematodes reached maturity, significant upregulation of MUC3, MUC4, resistin‐like molecule β (Relmβ) and 3O‐sulfotransferase (3ST)1 was observed, while 3ST2 expression levels increased after the majority of the worms were expelled from the intestine. Similar alterations of glycosylation‐related gene expression were also observed in mast‐cell‐deficient Ws/Ws rats, suggesting that mast cells in the epithelium are not relevant to the upregulation of these genes. The present finding that the expression level of each goblet cell‐ or glycosylation‐related gene was altered differently during the time course of infection indicates the progression of sequential qualitative changes in the mucus layer after infection.