Anticandidal Activity of Kalopanaxsaponin A: Effect on Proliferation, Cell Morphology, and Key Virulence Attributes of Candida albicans
Anticandidal Activity of Kalopanaxsaponin A: Effect on Proliferation, Cell Morphology, and Key Virulence Attributes of Candida albicans
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Kalopanaxsaponin A 的抗念珠菌活性：对白色念珠菌增殖、细胞形态和关键毒力属性的影响
DOI：
10.3389/fmicb.2019.02844
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发表时间：
2019-12-03
影响因子：
5.2
通讯作者：
Li, Hongchun
中科院分区：
文献类型：
--
作者：
Li, Ying;Shan, Mingzhu;Li, Hongchun
背景白念珠菌的致病性与多种毒力因子有关，包括粘附于上皮细胞或粘膜表面、芽管形成、菌丝形态发生、耐药生物膜形成等，本研究旨在探讨刺楸皂苷A（Kalopanaxsaponin A，KPA）对白念珠菌毒力的影响。白色念珠菌方法用KPA对C.白色念珠菌的特征在于通过XTT还原试验和荧光显微镜观察。采用GC/MS联用系统和BioTek Synergy 2荧光分光光度法进一步探讨其作用机理。采用秀丽隐杆线虫C.体内白念珠菌感染模型。结果KPA对不同基因型C.白色念珠菌该化合物对C.的粘附、形态转变和生物膜形成有显着影响。白色念珠菌荧光显微镜和GC/MS分析结果表明，KPA通过调节Dpp 3的表达，促进法尼醇的分泌，降低细胞内cAMP水平，从而抑制细胞形态转变和生物膜形成。值得注意的是，KPA在体内显示出低毒性和产生耐药性的可能性低。结论KPA对C.提示其可能成为临床治疗念珠菌病的一种潜在选择。
Background The pathogenicity of Candida albicans is attributed to various virulence factors including adhesion to the surface of epithelial cells or mucosa, germ tube formation, hyphal morphogenesis, development of drug resistant biofilms, and so on. The objective of this study was to investigate the effects of Kalopanaxsaponin A (KPA) on the virulence of C. albicans. Methods The effect of KPA on the virulence of C. albicans was characterized by an XTT reduction assay and fluorescent microscopic observation. The action mechanism was further explored using GC/MS system and BioTek Synergy2 spectrofluorophotometry. The cytotoxicity and therapeutic effect of KPA were evaluated by the Caenorhabditis elegans-C. albicans infection model in vivo. Results The minimum inhibitory concentration (MIC) of KPA was 8 similar to 16 mu g/mL for various genotypes of C. albicans. The compound was identified as having remarkable effect on the adhesion, morphological transition and biofilm formation of C. albicans. The results of fluorescent microscopy and GC/MS system suggested that KPA could promote the secretion of farnesol by regulating the expression of Dpp3 and decrease the intracellular cAMP level, which together inhibited morphological transition and biofilm formation. Notably, KPA showed low toxicity in vivo and a low possibility of developing resistance. Conclusion Our results demonstrated that KPA had remarkable efficacy against C. albicans pathogenicity, suggesting that it could be a potential option for the clinical treatment of candidiasis.