Effects of vertebral bone fragility and bone formation rate on the mineralization levels of cancellous bone from white females
Effects of vertebral bone fragility and bone formation rate on the mineralization levels of cancellous bone from white females
复制标题
DOI：
10.1016/s8756-3282(02)00975-4
复制
发表时间：
2003-03-01
期刊：
影响因子：
4.1
通讯作者：
Parfitt, AM
中科院分区：
文献类型：
--
作者：
Ciarelli, TE;Fyhrie, DP;Parfitt, AM
使用背散射电子显微镜研究白色女性（N = 49）人髂骨松质骨的矿化水平。通过使用已知校准标准将骨像素灰度级转换为原子序数（Z）来评估矿化水平。数据集由正常和椎骨骨折受试者的骨活检组成，这些受试者在各自的组内具有高或低的骨形成率（BFR）值（骨折/低BFR，N = 12;骨折/高BFR，N = 10;正常/低BFR，N = 12;正常/高BFR，N = 15）。定量测定每个样本的以下三种矿化指标：总体平均矿化（Z（平均值））、小梁内部深处的小梁包矿化（Z（深度））和浅表外部包矿化（Z（浅表））。双向方差分析显示，高BFR组的Z（浅表）显著低于低BFR组[平均值（SD）10.383（0.270）vs. 10.563（0.289）]，且无显著相互作用。BFR对Z（平均值）或Z（深度）没有影响。对于汇总数据，Z（深部）显著高于Z（浅表）[10.866（0.242）vs. 10.471（0.291）]。正常人和椎体骨折者之间的Z（平均值）、Z（深度）或Z（表面）无显著差异，但骨折组矿化测量的标准差至少是正常组的两倍。频率直方图显示两组具有根本不同的矿化分布。正常组表现出以平均值为中心的典型高斯分布，骨折组的分布是双峰的，峰值出现在正常组分布的高尾或低尾。我们假设低矿化和高矿化模式都可能对骨材料性能产生不良影响，低矿化水平导致刚度和强度降低，高矿化导致断裂韧性降低。矿化差异可能影响单个元素的强度和刚度的程度进行了估计。骨折组中矿化测量的较高标准差可能反映了无法正确调节骨小梁水平应力和应变。正向逐步回归分析显示，Ob.S/OS与Z（表面）和Z（平均值）之间存在显著相关性，表明成骨细胞可能在调节矿化中起重要作用。(C)2003 Elsevier Science（美国）。All rights reserved.
Back-scattered electron microscopy was used to study mineralization levels of human iliac cancellous bone of white females (N = 49). Mineralization levels were assessed by converting bone pixel grayscale levels to atomic number (Z) using known calibration standards. The data set consisted of bone biopsies from normal and vertebral fracture subjects that had either high or low values for bone formation rate (BFRs) within their respective groups (fracture/low BFRs, N = 12; fracture/high BFRs, N = 10; normal/low BFRs, N = 12; normal/high BFRs, N = 15). The following three measures of mineralization were quantitatively determined for each specimen: an overall mean mineralization (Z(mean)), the mineralization of trabecular packets deep within the interior of trabeculae (Z(deep)), and the mineralization of superficial exterior packets (Z(superficial)). Two-way analysis of variance revealed that the high BFRs group had a significantly lower Z(superficial) than the low BFRs, group [mean (SD) 10.383 (0.270) vs. 10.563 (0.289)], and there was no significant interaction. BFRs had no effect on Z(mean) or Z(deep). For the pooled data, Z(deep) was significantly higher than Z(superficial) [10.866 (0.242) vs. 10.471 (0.291)]. There was no significant difference in Z(mean), Z(deep), or Z(superficial) between normals and those with vertebral fracture, but the standard deviations of the mineralization measures in the fracture group were at least double that of the normal group. Frequency histograms show that the two groups have fundamentally different mineralization distributions. The normal group demonstrates typical Gaussian distributions centered around the mean, and the distributions of the fracture group are bimodal, with peaks occurring at either the high or low tails of the distributions of the normal group. We hypothesize that both low and high patterns of mineralization might detrimentally affect bone material properties, with low mineralization levels causing reduced stiffness and strength and high mineralization resulting in reduced fracture toughness. The degree to which the mineralization differences may affect strength and stiffness of individual elements is estimated. The higher standard deviations of mineralization measures in the fracture group may reflect an inability to properly regulate trabecular level stress and strain. Forward stepwise regression analysis showed significant relationships between Ob.S/OS and both Z(superficial) and Z(mean), suggesting that the osteoblast may play an important role in regulating mineralization. (C) 2003 Elsevier Science (USA). All rights reserved.