Retinal-specific ATP-binding cassette transporter (ABCR/ABCA4) is expressed at the choroid plexus in rat brain
Retinal-specific ATP-binding cassette transporter (ABCR/ABCA4) is expressed at the choroid plexus in rat brain
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DOI：
10.1111/j.1471-4159.2004.02941.x
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发表时间：
2005-03-01
影响因子：
4.7
通讯作者：
Terasaki, T
中科院分区：
文献类型：
--
作者：
Bhongsatiern, J;Ohtsuki, S;Terasaki, T
ATP结合盒（ABC）转运蛋白A4是ABC转运蛋白亚家族A的成员，据报道其仅在视网膜中表达。相反，以前的报告表明ABCA 4和CNS功能之间可能存在关系。本研究的目的是研究ABCA 4 mRNA和蛋白在大鼠脑内的定位。原位杂交分析显示ABCA 4 mRNA定位于侧脑室。RT-PCR检测了ABCA 4在离体大鼠脉络丛和条件永生化大鼠脉络丛上皮细胞（TR-CSFB）中的mRNA表达。此外，ABCA 4蛋白也检测到在离体大鼠脉络丛约250 kDa的蛋白质通过蛋白质印迹分析，其表观分子大小减少N-糖苷酶F处理。这些结果表明，糖基化ABCA 4蛋白在大鼠脉络丛上皮细胞中表达。ABCA 4可能在血液-脑脊液屏障的功能中发挥作用并影响CSF状况。
ATP-binding cassette (ABC) transporter A4 is a member of the ABC transporter subfamily A which has been reported to be exclusively expressed in the retina. In contrast, a previous report has suggested a possible relationship between ABCA4 and CNS function. The purpose of the present study was to investigate the localization of ABCA4 mRNA and protein in rat brain. In situ hybridization analysis revealed that ABCA4 mRNA was localized in the lateral ventricles. RT-PCR analysis detected ABCA4 mRNA in isolated rat choroid plexus and conditionally immortalized rat choroid plexus epithelial cells (TR-CSFB). Furthermore, ABCA4 protein was also detected in the isolated rat choroid plexus at about 250 kDa by western blot analysis, and its apparent molecular size was reduced by N-glycosidase F treatment. These results suggest that glycosylated ABCA4 protein is expressed in rat choroid plexus epithelial cells. ABCA4 may play a role in the function of the blood-cerebrospinal fluid barrier and affect CSF conditions.