Interaction of Sendai viral F, HN, and M proteins with host cytoskeletal and lipid components in Sendai virus-infected BHK cells.
Interaction of Sendai viral F, HN, and M proteins with host cytoskeletal and lipid components in Sendai virus-infected BHK cells.
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DOI：
10.1006/viro.1995.1308
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发表时间：
1995-06
期刊：
影响因子：
3.7
通讯作者：
C. Sanderson;R. Avalos;A. Kundu;D. Nayak
中科院分区：
文献类型：
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作者：
C. Sanderson;R. Avalos;A. Kundu;D. Nayak
我们研究了仙台病毒融合蛋白（F）、血凝素/神经氨酸酶（H/N）和基质蛋白（M）与仙台病毒感染的BHK细胞中宿主细胞骨架和脂质组分的相互作用，使用两种非离子去污剂Triton X-100（TX-100）和辛基葡萄糖苷（OG）。我们的研究结果表明，虽然M蛋白获得的TX-100和OG提取的阻力，F和HN表现出不溶性只有TX-100，但不OG。此外，在高盐（1 M NaCl）的存在下，M而不是F或HN变得可溶于TX-100。I型（F）和II型（HN）病毒糖蛋白在胞外转运的后期获得TX-100不溶性，因为它们获得了内H抗性。此外，通过浮选分析，与抗TX-100的M相比，TX-100不溶性F和HN表现出更轻的密度。使用单独表达仙台病毒HN、F或M蛋白的重组牛痘病毒，我们观察到每种病毒蛋白（F、HN或M）在不存在其他病毒组分的情况下能够独立地获得TX-100不溶性。这些结果表明，虽然仙台病毒F和HN与TX-100不溶性脂质结合，但M蛋白与TX-100不溶性细胞骨架组分离子结合，而不与TX-100不溶性脂质结合。
We have studied the interaction of Sendai viral fusion (F), hemagglutinin/neuraminidase (H/N), and matrix (M) proteins with host cytoskeletal and lipid components in Sendai virus-infected BHK cells using two nonionic detergents Triton X-100 (TX-100) and octyl glucoside (OG). Our results show that while M protein acquired resistance to both TX-100 and OG extraction, F and HN exhibited insolubility only to TX-100 but not to OG. Furthermore, in the presence of high salt (1 M NaCl), M, but not F or HN, became TX-100 soluble. Both type I (F) and type II (HN) viral glycoproteins acquired TX-100 insolubility at a late stage during exocytic transport as they acquired endo H resistance. In addition, TX-100 insoluble F and HN exhibited a lighter density compared to TX-100 resistant M by flotation analysis. Using recombinant vaccinia viruses that express Sendai virus HN, F, or M protein individually, we observed that each viral protein (F, HN, or M) was independently capable of acquiring TX-100 insolubility in the absence of other viral components. These results would indicate that while Sendai viral F and HN became bound to TX-100 insoluble lipids, M protein bound ionically to TX-100 insoluble cytoskeletal components and not to TX-100 insoluble lipids.