Cross-linking of phosphatidylethanolamine neighbors with dimethylsuberimidate is sensitive to the lipid phase.
Cross-linking of phosphatidylethanolamine neighbors with dimethylsuberimidate is sensitive to the lipid phase.
复制标题
磷脂酰乙醇胺邻居与二甲基亚胺酸酯的交联对脂质相敏感。
DOI：
10.1016/0005-2736(89)90470-7
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Welti,R
中科院分区：
文献类型：
--
作者：
Roth,MR;Avery,RB;Welti,R
辛二酰亚胺酸二甲酯与二棕榈酰磷脂酰乙醇胺、二肉豆蔻酰磷脂酰乙醇胺、二月桂酰磷脂酰乙醇胺和二反油酰磷脂酰乙醇胺的水分散体在pH 10和pH 8下反应。在每种脂质的凝胶-流体相转变上方形成的脒二聚体的量比该转变下方的多约四倍。通过顺式石蜡酸的稳态荧光各向异性测量，每种磷脂酰乙醇胺的转变温度在 pH 10 时低于 pH 8 或在水中。辛二酰亚胺酸二甲酯区分流体相和凝胶相中磷脂酰乙醇胺的能力应允许使用该试剂来识别凝胶或流体脂质相内的磷脂酰乙醇胺种类。
Dimethylsuberimidate was reacted with aqueous dispersions of dipalmitoylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, and dielaidoylphosphatidylethanolamine at pH 10 and at pH 8. The amount of amidine dimer formation was about four times greater above the gel-to-fluid phase transition of each lipid than below the transition. The transition temperature of each phosphatidylethanolamine, measured by steady-state fluorescence anisotropy ofcis-parinaric acid, was lower at pH 10 than at pH 8 or in water. The ability of dimethylsuberimidate to discriminate between phosphatidylethanolamines in the fluid and gel phases should allow use of this reagent to identify phosphatidylethanolamine species within the gel or fluid lipid phase.