Discovery of Tick-Borne Karshi Virus Implies Misinterpretation of the Tick-Borne Encephalitis Virus Seroprevalence in Northwest China.
Discovery of Tick-Borne Karshi Virus Implies Misinterpretation of the Tick-Borne Encephalitis Virus Seroprevalence in Northwest China.
复制标题
DOI：
10.3389/fmicb.2022.872067
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
尽管蜱传脑炎病毒（TBEV）的人间病例很少，但在中国西北部的新疆维吾尔自治区，TBEV血清阳性率在人和动物中报告很高。本研究从新疆地区的亚洲璃眼蜱中分离到Karshi病毒。它属于黄病毒科的黄病毒属，与TBEV密切相关。KSIV感染来自人类、其他哺乳动物和蜱的细胞系，并在乳鼠中引起脑炎。蜱类中检出KSIV的最低感染率较高（4.96%）。绵羊和旱獭也发生了KSIV感染，导致抗体阳性率分别为2.43%和2.56%。我们进一步发现，在KSIV抗体阳性的动物血清样本中，13.9%的TBEV暴露显示出与KSIV的交叉反应，11.1%的KSIV感染导致与TBEV的交叉反应; 8.3%的人可能同时暴露于两种病毒（或可能感染其中一种病毒，并与另一种病毒呈现交叉反应）。结果显示，新疆蜱中KSIV的流行率很高，表明动物暴露于KSIV和TBEV。这一发现意味着对以前研究中人类和动物中TBEV血清阳性率高的误解。有必要建立区分KSIV和TBEV的检测方法，并对KSIV和TBEV在中国西北地区的流行和发病率进行深入调查，这将有助于我们为治疗由蜱传病毒病原体（如KSIV）引起的新发疾病做好准备。
Despite few human cases of tick-borne encephalitis virus (TBEV), high rates of TBEV seroprevalence were reported among humans and animals in Xinjiang Uygur Autonomous Region in Northwestern China. In this study, the Karshi virus (KSIV) was identified and isolated from Hyalomma asiaticum ticks in Xinjiang. It belongs to the genus Flavivirus of the family Flaviviridae and is closely related to TBEV. KSIV infects cell lines from humans, other mammals and ticks, and causes encephalitis in suckling mice. High minimum infection rates (4.96%) with KSIV were detected among tick groups. KSIV infections have occurred in sheep and marmots, resulting in antibody-positive rates of 2.43 and 2.56%, respectively. We further found that, of the KSIV antibody-positive serum samples from animals, 13.9% had TBEV exposure showing cross-reaction to KSIV, and 11.1% had KSIV infection resulting in cross-reaction to TBEV; 8.3% were likely to have co-exposure to both viruses (or may be infected with one of them and present cross-reactivity with the other). The results revealed a substantial KSIV prevalence among ticks in Xinjiang, indicating exposure of animals to KSIV and TBEV. The findings implied misinterpretation of the high rates of TBEV seroprevalence among humans and animals in previous studies. There is a need to develop detection methods to distinguish KSIV from TBEV and to perform an in-depth investigation of KSIV and TBEV prevalence and incidence in Northwestern China, which would enhance our preparation to provide medical treatment of emerging diseases caused by tick-borne viral pathogens such as KSIV.