3 DIFFERENT MESSENGER-RNAS ENCODING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR
3 DIFFERENT MESSENGER-RNAS ENCODING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR
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DOI：
10.1073/pnas.87.22.8702
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发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
NAGATA, S
中科院分区：
文献类型：
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作者：
FUKUNAGA, R;SETO, Y;NAGATA, S
以小鼠粒细胞集落刺激因子（G-CSF）受体cDNA为探针，从人U937白血病细胞和胎盘cDNA文库中分离到3个G-CSF受体cDNA。人G-CSF受体含有813个氨基酸，与鼠G-CSF受体具有显著的同源性（62.5%），由胞外、跨膜和胞质结构域组成。在新鉴定的生长因子受体家族成员中发现的WSXWS基序也存在于人G-CSF受体的胞外结构域中。克隆的cDNA在猴COS细胞中的表达产生了能够以高亲和力（Kd，550 pM）特异性结合G-CSF的蛋白质。还鉴定了另外两类人G-CSF受体，其中一类缺失跨膜结构域，似乎编码分泌的可溶性受体。第三类G-CSF受体包含一个27个氨基酸的插入胞质结构域，并在胎盘中高度表达。
Three cDNAs for the human granulocyte colony-stimulating factor (G-CSF) receptor were isolated from the cDNA libraries of human U937 leukemia cells and placenta by using a murine G-CSF receptor cDNA as the probe. The human G-CSF receptor containing 813 amino acids had a marked homology (62.5%) with its murine counterpart and consisted of extracellular, transmembrane, and cytoplasmic domains. The WSXWS motif found in members of the newly identified growth factor receptor family was also present in the extracellular domain of the human G-CSF receptor. Expression of the cloned cDNA in monkey COS cells gave rise to a protein that could specifically bind G-CSF with a high affinity (Kd, 550 pM). Two other classes of the human G-CSF receptor were also identified, one of which had a deletion of the transmembrane domain and seemed to encode a secreted, soluble receptor. The third class of the G-CSF receptor contained a 27-amino acid insertion of the cytoplasmic domain and was highly expressed in placenta.