Comparison of detachment procedures for direct counts of bacteria associated with sediment particles, plant litter and epiphytic biofilms
Comparison of detachment procedures for direct counts of bacteria associated with sediment particles, plant litter and epiphytic biofilms
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DOI：
10.3354/ame027029
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发表时间：
2002-02-18
影响因子：
1.4
通讯作者：
Gessner, MO
中科院分区：
文献类型：
--
作者：
Buesing, N;Gessner, MO
细菌细胞的有效分离对于用各种分析方法评估自然和技术系统(如污水处理厂)中的细菌丰度、生物量和群落组成至关重要。对于哪种类型的基质，哪种方法效果最好，还没有达成一致意见。我们测试了4种分离仪器对自然水生系统中与叶凋落物、沉积物和附生生物膜相关的细菌的释放效果。与简单的涡流处理相比，大多数仪器的处理显著增加了细菌数量和生物体积(增加了2.7到7倍)。然而，不同的分离装置释放的细菌细胞的数量和生物量都有显著的差异。利用落叶和附生生物膜，超声波探头处理释放的细菌细胞比最低效的仪器多10倍和4倍。胃型搅拌器对沉积物样本的效果最好，释放的细菌数量是效率最低的搅拌器的3倍。分离器械和处理时间均不影响细菌形态类型的组成。这些结果表明，当要确定细菌的绝对丰度或生物量时，适当的分离装置的选择关键取决于所检查的底物类型。在细菌群落结构的定性分析中，所选择的装置似乎不那么重要。
Efficient detachment of bacterial cells is crucial for assessing bacterial abundance, biomass and community composition in natural and technical systems (e.g. sewage plants) by a wide range of analytical methods. There is no agreement on which procedure gives the best results with which type of substratum. We tested the effect of 4 detachment instruments on the release of bacteria associated with leaf litter, sediment and epiphytic biofilms from a natural aquatic system. Treatment with most instruments increased bacterial counts and biovolumes significantly compared to simple vortexing (by 2.7 to 7x). However, both the numbers and biovolume of released bacterial cells varied significantly between detachment devices. With leaf litter and epiphytic biofilms, an ultrasonic probe treatment released 10 and 4 times more bacterial cells than the most inefficient instrument. A stomacher-type blender gave the best results for sediment samples, releasing 3 times higher numbers of bacteria than the least efficient instrument. Neither the detachment instrument nor the treatment time affected the composition of bacterial morphotypes. These results indicate that the choice of the appropriate detachment device depends critically on the type of substratum examined, when the absolute abundance or biomass of bacteria is to be determined. In qualitative analyses of bacterial community structure the chosen device appears to be less important.