Overexpression of an F-box protein gene disrupts cotyledon vein patterning in Arabidopsis
Overexpression of an F-box protein gene disrupts cotyledon vein patterning in Arabidopsis
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F-box 蛋白基因的过度表达会破坏拟南芥子叶静脉模式
DOI：
10.1016/j.plaphy.2016.02.012
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发表时间：
2016-05-01
影响因子：
6.5
通讯作者：
Zhu, Jian
中科院分区：
文献类型：
--
作者：
Cui, Xianghuan;Xu, Xiaofeng;Zhu, Jian
植物的维管构型是复杂的。然而，血管图案化的详细分子机制仍不清楚。本研究利用3‘-RACE技术分离了拟南芥F-box基序基因FBXL。该基因编码1407个核苷酸，编码468个氨基酸残基。氨基酸序列分析表明，该基因编码一个蛋白质，N端有F-box基序，中间有LRRS基序，C端有FBD基序。为了解FBXL基因的功能，构建了FBXL启动子-fl-葡萄糖醛酸酶(GUS)和35S启动子-FBXL载体。GUS表达分析表明，FBXL在根、茎、叶和花序的维管组织中特异表达。在拟南芥中过表达的FBXL在子叶中表现出异常的脉序模式。此外，FBXL的表达不受外源生长素的诱导，其转录积累与内源生长素的分布不重叠。这些结果表明，FBXL可能通过不依赖生长素的途径参与子叶脉纹的形成。(C)2016年爱思唯尔·马森SAS。版权所有。
Plant vascular patterning is complex. However, the detailed molecular mechanism of vascular patterning is still unknown. In this study, FBXL, an Arabidopsis F-box motif gene, was isolated by using 3' rapid amplification of cDNA ends (RACE) technique. The gene contained a coding sequence of 1407 nucleotides coding 468 amino acid residues. Amino acid sequence analysis revealed that the gene encoded a protein harboring an F-box motif at the N terminus, an LRRs motif in the middle, and an FBD motif at the C terminus. FBXL promoter-fl-glucuronidase (GUS) and 35S promoter-FBXL vectors were constructed and transformed into Arabidopsis thaliana to understand the function of the FBXL gene. GUS expression analysis indicated that FBXL was specifically expressed in the vascular tissues of the root, stem, leaf, and inflorescence. FBXL overexpression in Arabidopsis displayed an abnormal venation pattern in cotyledons. Furthermore, FBXL expression was not induced by exogenous auxin and its transcript accumulation did not overlap with the distribution of endogenous auxin. These results suggested that FBXL may be involved in cotyledon vein patterning via auxin -independent pathway. (C) 2016 Elsevier Masson SAS. All rights reserved.