A Secreted BBE-Like Enzyme Acting as a Drug-Binding Efflux Carrier Confers Microbial Self-Resistance to Mitomycin C.
A Secreted BBE-Like Enzyme Acting as a Drug-Binding Efflux Carrier Confers Microbial Self-Resistance to Mitomycin C.
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DOI：
10.1021/acs.orglett.4c00042
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发表时间：
2024-02
期刊：
影响因子：
5.2
通讯作者：
Xiaorong Chen;Rui He;Aiai Sun;Jinyue Pu;Hai-Xue Pan;Gong-Li Tang
中科院分区：
文献类型：
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作者：
Xiaorong Chen;Rui He;Aiai Sun;Jinyue Pu;Hai-Xue Pan;Gong-Li Tang
小檗碱桥酶（BBE）类黄素蛋白因其催化多种氧化反应的能力而引起人们的广泛关注。在这里，我们表明，MitR，分泌的BBE样酶，功能作为一个特殊的药物结合外排蛋白醌还原酶演变。此外，这种蛋白质提供了自我抵抗其宿主对DNA烷化剂丝裂霉素C的独特策略，其特点是独立进行药物结合和外排。
The berberine bridge enzyme (BBE)-like flavoproteins have attracted continuous attention for their capability to catalyze various oxidative reactions. Here we demonstrate that MitR, a secreted BBE-like enzyme, functions as a special drug-binding efflux protein evolved from quinone reductase. Moreover, this protein provides self-resistance to its hosts toward the DNA-alkylating agent mitomycin C with a distinctive strategy, featured by independently performing drug binding and efflux.