Microtubule-binding properties of dynactin p150 expedient for dynein motility
Microtubule-binding properties of dynactin p150 expedient for dynein motility
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DOI：
10.1016/j.bbrc.2005.11.145
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发表时间：
2006-02-03
影响因子：
3.1
通讯作者：
Toyoshima, YY
中科院分区：
文献类型：
--
作者：
Kobayashi, T;Shiroguchi, K;Toyoshima, YY
动力蛋白是一种异寡聚体蛋白复合体，在动力蛋白的细胞内转运中起着重要作用。表达的Dynactin P150的N-末端片段以1：1的比例与微管结合，与Dynein茎头的结合无关。单分子观察表明，这些碎片以布朗运动的方式在微管上运动。当动力蛋白-动力蛋白复合体在微管上运动时，P150可以支持动力蛋白与微管保持接触，不干扰动力蛋白的运动，因此，动力蛋白-动力蛋白复合体可以有效地实现货物的远距离运输。(C)2005 Elsevier Inc.保留所有权利。
Dynactin is a hetero-oligomeric protein complex that has an important role in dynein-based intracellular transport. The expressed N-terminal fragments of dynactin p150 bound to microtubules in the ratio of one to one tubulin dimer, independent from the binding of dynein stalk head. Single molecule observation revealed that these fragments moved around on microtubules by Brownian motion. When the dynein-dynactin complex moves on microtubules, p150 can support dynein to maintain contact with microtubules and does not interfere with the motility of dynein, and thus, the dynein-dynactin complex can efficiently achieve long-distance carriage of the cargo. (c) 2005 Elsevier Inc. All rights reserved.