Mosaic patterns of B-vitamin synthesis and utilization in a natural marine microbial community
Mosaic patterns of B-vitamin synthesis and utilization in a natural marine microbial community
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DOI：
10.1111/1462-2920.14133
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发表时间：
2018-08-01
影响因子：
5.1
通讯作者：
Moran, Mary Ann
中科院分区：
文献类型：
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作者：
Gomez-Consarnau, Laura;Sachdeva, Rohan;Moran, Mary Ann
水生环境含有大量微生物群落，它们的协同相互作用介导了包括维生素在内的主要和微量营养素的循环。维生素B是许多生物体不能合成的必需辅酶。因此，他们之间的交换从头合成和营养缺陷型预计将发挥重要作用的微生物财团和解释一些时间和空间的变化观察到的多样性。在这项研究中，我们分析了一个天然海洋微生物群落的元转录组，在一年内每季度进行一次采样，试图确定潜在的主要B族维生素合成者和消费者。转录组学数据显示，最具代表性的类群主导了一些B族维生素合成基因的表达，但缺乏其他转录本。例如，维生素B-12合成的表达主要由红球藻目控制，而维生素B-7合成的转录本主要由黄杆菌属代表。相比之下，占群落不到4%的细菌群（例如，Verrucomicrobia）占维生素B-1合成转录本的大部分。此外，在白天采集的样品中，环境维生素B-1浓度较高，并且与叶绿素a浓度呈正相关。我们的分析支持这一假设，即通过B族维生素合成和交换的代谢相互依赖性的马赛克是有助于塑造自然界中微生物群落的关键过程。
Aquatic environments contain large communities of microorganisms whose synergistic interactions mediate the cycling of major and trace nutrients, including vitamins. B-vitamins are essential coenzymes that many organisms cannot synthesize. Thus, their exchange among de novo synthesizers and auxotrophs is expected to play an important role in the microbial consortia and explain some of the temporal and spatial changes observed in diversity. In this study, we analyzed metatranscriptomes of a natural marine microbial community, diel sampled quarterly over one year to try to identify the potential major B-vitamin synthesizers and consumers. Transcriptomic data showed that the best-represented taxa dominated the expression of synthesis genes for some B-vitamins but lacked transcripts for others. For instance, Rhodobacterales dominated the expression of vitamin-B-12 synthesis, but not of vitamin-B-7, whose synthesis transcripts were mainly represented by Flavobacteria. In contrast, bacterial groups that constituted less than 4% of the community (e.g., Verrucomicrobia) accounted for most of the vitamin-B-1 synthesis transcripts. Furthermore, ambient vitamin-B-1 concentrations were higher in samples collected during the day, and were positively correlated with chlorophyll-a concentrations. Our analysis supports the hypothesis that the mosaic of metabolic interdependencies through B-vitamin synthesis and exchange are key processes that contribute to shaping microbial communities in nature.