ASSOCIATION BETWEEN A LARGE PLASMID AND 15-KILODALTON TO 17-KILODALTON ANTIGENS IN VIRULENT RHODOCOCCUS-EQUI
ASSOCIATION BETWEEN A LARGE PLASMID AND 15-KILODALTON TO 17-KILODALTON ANTIGENS IN VIRULENT RHODOCOCCUS-EQUI
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DOI：
10.1128/iai.59.11.4056-4060.1991
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发表时间：
1991-11-01
影响因子：
3.1
通讯作者：
TSUBAKI, S
中科院分区：
文献类型：
--
作者：
TAKAI, S;SEKIZAKI, T;TSUBAKI, S
在免疫印迹中显示15- 17-kDa抗原的马红球菌菌株被发现在小鼠中是有毒的。 目的研究强毒沙门氏菌对这些抗原的特异性基因。equi，我们比较了10株R.等。 所有显示15至17 kDa抗原的菌株均含有约85 kbp的大质粒，在小鼠中具有毒性;然而，其余菌株缺乏抗原和大质粒，在小鼠中无毒力。 通过在38 ℃下反复传代消除大质粒的强毒株ATCC 33701和L1的突变体缺乏15- 17-kDa抗原，并且在小鼠中显示出致命性的显著降低。 这些结果表明，85-kbp质粒的存在可能是必不可少的毒力和表达的15- 17-kDa抗原的R。equi，并为早期的观察提供了支持，即新鲜分离的R.马杀死了小鼠，而实验室适应的菌株却没有。
Rhodococcus equi strains showing 15- to 17-kDa antigens in immunoblots were found to be virulent in mice. To study the genes specific to these antigens in virulent R. equi, we compared plasmid profiles, immunoblot profiles, and murine pathogenicity profiles of 10 strains of R. equi. All the strains showing 15- to 17-kDa antigens contained a large plasmid of approximately 85 kbp and were virulent in mice; however, the remaining strains lacked both the antigens and the large plasmid and were avirulent in mice. Mutants of virulent strains ATCC 33701 and L1, which were cured of the large plasmid by repeated passage at 38-degrees-C, lacked the 15- to 17-kDa antigens and showed a dramatic decrease in lethality in mice. These results suggested that the presence of an 85-kbp plasmid may be essential for virulence and expression of 15- to 17-kDa antigens of R. equi and offered support for earlier observations that freshly isolated strains of R. equi killed mice, whereas laboratory-adapted strains did not.