Chemical LTD in the CA1 field of the hippocampus from young and mature rats
Chemical LTD in the CA1 field of the hippocampus from young and mature rats
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DOI：
10.1046/j.1460-9568.1999.00769.x
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发表时间：
1999-10-01
影响因子：
3.4
通讯作者：
Gispen, WH
中科院分区：
文献类型：
--
作者：
Kamal, A;Ramakers, GMJ;Gispen, WH
在海马结构中，可以诱导两种形式的长时程突触可塑性，即长时程增强(LTP)和长时程抑制(LTD)，这两种形式都需要激活NMDA受体。有趣的是，LTP和LTD在成年动物中都有减少。最近，一种新的化学方案被描述出来，它在海马区的CA1区引发了LTD。20mU N-甲基-D-天冬氨酸作用3min后，诱发的突触反应持续稳定地下降。我们使用该方法在幼年和成年大鼠的海马片中诱导LTD，并表明这种形式的LTD对AP5敏感，并且可以被成年动物脑片中的蛋白磷酸酶抑制剂环孢素A阻断。与长时间低频刺激诱发的电LTD相比，幼年和成年大鼠化学性LTD的程度无明显差异。这些发现表明，在成年动物中，参与长时间突触抑制的细胞内信号转导级联仍然完好无损。
Within the hippocampal formation, two forms of long-lasting synaptic plasticity, long-term potentiation (LTP) and long-term depression (LTD), can be induced which require the activation of NMDA receptors. Interestingly, it has been shown that both LTP and LTD are reduced in adult animals. Recently, a new chemical protocol has been described which elicits LTD in the CA1 field of the hippocampus. Application of 20 mu M NMDA for 3 min results in a stable and long-lasting decrease in the evoked synaptic responses. We used this protocol to induce LTD in hippocampal slices from young and adult rats and show that this form of LTD is AP5-sensitive and can be blocked by the protein phosphatase inhibitor cyclosporin A in slices from adult animals. In contrast to electrical LTD (induced by prolonged low frequency stimulation), the extent of chemical LTD was not different between the young and adult rats. These findings indicate that the intracellular signal transduction cascades involved in long-lasting synaptic depression are still intact in adult animals.