Histone Acetylation of Immune Regulatory Genes in Human Placenta in Association with Maternal Intake of Olive Oil and Fish Consumption
Histone Acetylation of Immune Regulatory Genes in Human Placenta in Association with Maternal Intake of Olive Oil and Fish Consumption
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DOI：
10.3390/ijms20051060
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发表时间：
2019-03-01
影响因子：
5.6
通讯作者：
Potaczek, Daniel P.
中科院分区：
文献类型：
--
作者：
Acevedo, Nathalie;Frumento, Paolo;Potaczek, Daniel P.
母亲的饮食改变了后代的表观遗传程序，这是现代社会免疫失调的一个潜在关键因素。我们先前发现，产前补充鱼油会影响新生儿T细胞组蛋白乙酰化，这些基因与获得性免疫有关，包括PRKCZ、IL13和Tbx21。在这项研究中，我们用染色质免疫沉淀法检测了173例足月胎盘的H3和H4组蛋白乙酰化水平，这些胎盘是在预期出生队列Aladdin中收集的，其中详细记录了生活方式和饮食的信息。在具有人类智商的家庭中，怀孕期间经常使用橄榄油与FOXP3(p=0.004)、IL10RA(p=0.008)和IL7R(p=0.007)启动子H3乙酰化增加有关，经后代性别调整后仍显著。此外，母体食用鱼类与雌性后代胎盘中CD14基因H4乙酰化增加相关(p=0.009)。总之，产前摄入橄榄油可以影响免疫调节基因中胎盘组蛋白的乙酰化，证实了之前观察到的橄榄油多酚的乙酰化作用。与鱼类消费的关联可能涉及鱼油中存在的-3多不饱和脂肪酸。经常在饮食中加入鱼或橄榄油的母亲胎盘中组蛋白乙酰化的改变可能会影响新生儿的免疫启动。
Maternal diet modifies epigenetic programming in offspring, a potentially critical factor in the immune dysregulation of modern societies. We previously found that prenatal fish oil supplementation affects neonatal T-cell histone acetylation of genes implicated in adaptive immunity including PRKCZ, IL13, and TBX21. In this study, we measured H3 and H4 histone acetylation levels by chromatin immunoprecipitation in 173 term placentas collected in the prospective birth cohort, ALADDIN, in which information on lifestyle and diet is thoroughly recorded. In anthroposophic families, regular olive oil usage during pregnancy was associated with increased H3 acetylation at FOXP3 (p = 0.004), IL10RA (p = 0.008), and IL7R (p = 0.007) promoters, which remained significant after adjustment by offspring gender. Furthermore, maternal fish consumption was associated with increased H4 acetylation at the CD14 gene in placentas of female offspring (p = 0.009). In conclusion, prenatal olive oil intake can affect placental histone acetylation in immune regulatory genes, confirming previously observed pro-acetylation effects of olive oil polyphenols. The association with fish consumption may implicate -3 polyunsaturated fatty acids present in fish oil. Altered histone acetylation in placentas from mothers who regularly include fish or olive oil in their diets could influence immune priming in the newborn.