Early-stage osseointegration capability of a submicrofeatured titanium surface created by microroughening and anodic oxidation.
Early-stage osseointegration capability of a submicrofeatured titanium surface created by microroughening and anodic oxidation.
复制标题
通过微粗糙化和阳极氧化形成的亚微米钛表面的早期骨整合能力。
DOI：
10.1111/j.1600-0501.2012.02507.x
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发表时间：
2013
影响因子：
4.3
通讯作者：
Ogawa,Takahiro
中科院分区：
文献类型：
--
作者：
Yamada,Masahiro;Ueno,Takeshi;Minamikawa,Hajime;Ikeda,Takayuki;Nakagawa,Kaori;Ogawa,Takahiro
纳米/亚微米形态特征在骨整合过程中的作用在很大程度上是未知的。本研究报告创建一个独特的亚微观特征的钛表面相结合的阳极氧化和喷砂，并确定如何增加这种亚微观特征的microroughened表面影响的早期过程的osteointegration.Materials和methodsNonmicroroughened种植体的制备通过加工Ti-6Al-4V合金在圆柱形（1毫米直径和2毫米长）。通过喷砂机加工植入物制备微粗糙植入物，而通过喷砂植入物的阳极氧化产生亚微特征植入物。植入物被放置到大鼠股骨，并进行生物力学，界面和组织学分析，在1和2周后植入（n= 6）。ResultsThe submicrotopography的特点是由50-300 nm的结节和凹坑，除了其他亚微米级的不规则性完全形成的喷砂创建的微观结构。从第1周到第2周，亚微特征种植体的骨结合生物力学强度持续增加，但微粗糙种植体的骨结合生物力学强度没有增加。与非微粗糙表面相比，通常发现微粗糙表面和亚微特征表面的骨-植入物接触和骨体积显著增加，以及软组织干预减少。然而，有没有这些参数之间的微粗糙化表面和亚微特征表面的差异。在生物力学剪切测试后，亚显微特征植入物界面处的大面积骨组织保持完整，而显微粗糙化植入物-组织界面显示出沿植入物整个轴沿着的间隙，结论本研究表明，喷砂和阳极氧化相结合产生的亚微特征钛表面增强了早期阶段的强度。骨整合，主要是因为种植体骨组织对外力的抵抗力增加，而不是骨形态发生的调节。
ObjectiveThe role of nanoscale/submicron morphological features in the process of osseointegration is largely unknown. This study reports the creation of a unique submicrofeatured titanium surface by a combination of anodic oxidation and sandblasting and determines how the addition of this submicrofeature to a microroughened surface affects the early‐stage process of osseointegration.Materials and methodsNonmicroroughened implants were prepared by machining Ti‐6Al‐4V alloy in a cylindrical form (1 mm diameter and 2 mm long). Microroughened implants were prepared by sandblasting machined implants, while submicrofeatured implants were created by anodic oxidation of the sandblasted implants. Implants were placed into rat femurs and subjected to biomechanical, interfacial, and histological analyses at 1 and 2 weeks post‐implantation (n= 6).ResultsThe submicrotopography was characterized by 50–300 nm nodules and pits in addition to other submicron‐level irregularities formed entirely within the sandblast‐created microstructures. The biomechanical strength of osseointegration increased continuously from week 1 to 2 for the submicrofeatured implants but not for the microroughened implants. A significant increase in bone‐implant contact and bone volume, as well as a reduction in soft tissue intervention, were commonly found for the microroughened surface and the submicrofeatured surface compared with the nonmicroroughened surface. However, there were no differences in these parameters between the microroughened surface and the submicrofeatured surface. An extensive area of bone tissue at the submicrofeatured implant interface was retained intact after biomechanical shear testing, while the microroughened implant‐tissue interface showed a gap along the entire axis of the implant, leading to clear separation of the tissue during the shear procedure.ConclusionsThis study demonstrates that a submicrofeatured titanium surface created by a combination of sandblasting and anodic oxidation enhances the strength of early‐stage osseointegration, primarily because of the increased resistance of peri‐implant bone tissue against external force rather than modulation of bone morphogenesis.