Transcriptome analysis of embryonic and adult sensory axons reveals changes in mRNA repertoire localization
Transcriptome analysis of embryonic and adult sensory axons reveals changes in mRNA repertoire localization
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DOI：
10.1261/rna.2386111
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发表时间：
2011-01-01
期刊：
影响因子：
4.5
通讯作者：
Fawcett, James W.
中科院分区：
文献类型：
--
作者：
Gumy, Laura F.;Yeo, Giles S. H.;Fawcett, James W.
mRNA在感觉神经元的轴突中转运、定位和翻译。然而，很少有人知道完整的剧目存在于胚胎和成人感觉轴突的转录本，以及如何在发展过程中动态变化的mRNA池。在这里，我们使用了一个分隔室分离mRNA从纯胚胎和成人的感觉轴突没有非神经元或细胞体的污染。全基因组微阵列分析表明，以前不受重视的数量的转录本定位于感觉轴突，这一剧目的变化，在向成年期的发展。胚胎轴突富含编码细胞内相关蛋白的转录物，这些蛋白在轴突生长中起作用。令人惊讶的是，成年轴突富含编码在伤害感受中起作用的免疫分子的mRNA。此外，我们发现微管蛋白β 3（Tubb3）mRNA仅存在于胚胎轴突中，Tubb3在胚胎轴突中局部合成，而不是在成年神经元中运输，从而验证了我们的实验方法。总之，我们提供了第一个完整的目录胚胎和成人感觉轴突的mRNA。此外，我们表明，这个池的轴突mRNA的动态变化过程中的发展。这些数据为研究神经元发育过程中局部蛋白质合成在轴突再生和伤害感受中的作用提供了重要资源。
mRNAs are transported, localized, and translated in axons of sensory neurons. However, little is known about the full repertoire of transcripts present in embryonic and adult sensory axons and how this pool of mRNAs dynamically changes during development. Here, we used a compartmentalized chamber to isolate mRNA from pure embryonic and adult sensory axons devoid of non-neuronal or cell body contamination. Genome-wide microarray analysis reveals that a previously unappreciated number of transcripts are localized in sensory axons and that this repertoire changes during development toward adulthood. Embryonic axons are enriched in transcripts encoding cytoskeletal-related proteins with a role in axonal outgrowth. Surprisingly, adult axons are enriched in mRNAs encoding immune molecules with a role in nociception. Additionally, we show Tubulin-beta3 (Tubb3) mRNA is present only in embryonic axons, with Tubb3 locally synthesized in axons of embryonic, but not adult neurons where it is transported, thus validating our experimental approach. In summary, we provide the first complete catalog of embryonic and adult sensory axonal mRNAs. In addition we show that this pool of axonal mRNAs dynamically changes during development. These data provide an important resource for studies on the role of local protein synthesis in axon regeneration and nociception during neuronal development.