The effect of globopentaosylceramide on a depression model, mouse forced swimming.
The effect of globopentaosylceramide on a depression model, mouse forced swimming.
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球五糖神经酰胺对抑郁症模型（小鼠强迫游泳）的影响。
DOI：
10.1620/tjem.191.47
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
T. Sugiyama
中科院分区：
文献类型：
--
作者：
Y. Masuda;T. Sugiyama
我们研究了5-羟色胺能药物对小鼠强迫游泳的迟发效应，发现5-羟色胺1A拮抗剂NAN-190氢溴酸盐（NAN-190 hydrobromide，NAN-190）和5-羟色胺2激动剂R（-）-DO 1 hydrochloride（DO 1）在腹腔注射后6小时增加了小鼠典型的抗抑郁行为。采用离子交换、超滤和化学提取方法，从药物处理的小鼠血清中提取出对小鼠强迫游泳具有显著抗抑郁活性的物质。该物质被强烈认为是在其末端具有特定糖链结构GalNAcalpha 1 -3GalNAc的糖脂。事实上，另一种具有末端结构的糖脂globopentaosylceramide也对小鼠强迫游泳显示出显著的抗抑郁活性，但缺乏糖链结构的globotetraosylceramide没有。血清中的GalNAcalpha 1 - 3GalNAc-脂质反应性和典型的抗抑郁行为攀爬相应于NAN-190和DO 1的剂量而增加。这些发现清楚地表明，末端结构GalNAcalpha 1 -3GalNAc在globopentaosylceramide的抗抑郁活性中具有重要作用，并且5-羟色胺1A拮抗作用和5-羟色胺2激动作用增加了小鼠血清中具有末端结构的抗抑郁糖脂的产生。
We investigated the late effects of serotonergic drugs on mouse forced swimming and found that serotonin 1A antagonist NAN-190 hydrobromide (NAN-190) and serotonin 2 agonist R(-)-DO1 hydrochloride (DO1) increase the typical anti-depressive behavior climbing 6 hours after the intraperitoneal injection. With the use of ion exchanger, ultra filtration and chemical extraction methods, a substance having remarkable anti-depressive activity on mouse forced swimming was extracted from the serums of the mice treated with the drugs. The substance was strongly suggested to be a glycolipid having a specific sugar chain structure GalNAcalpha1-3GalNAc in its terminal. In fact, globopentaosylceramide, another glycolipid having the structure in the terminal also showed marked anti-depressive activity on mouse forced swimming, but globotetraosylceramide lacking the sugar chain structure did not. The GalNAcalpha1-3GalNAc-lipid reactivity in the serum and typical anti-depressive behavior climbing increased corresponding to the doses of NAN-190 and DO1. These findings clearly indicate that the terminal structure GalNAcalpha1-3GalNAc has the essential role in the anti-depressive activity of globopentaosylceramide, and that serotonin 1A antagonism and serotonin 2 agonism increase the production of an anti-depressive glycolipid having the terminal structure in mouse serum.
神经节苷脂支持神经视网膜细胞粘附。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Blackburn,CC;Swank-Hill,P;Schnaar,RL
通讯作者：
Schnaar,RL