Development of a lateral-flow assay (LFA) for rapid detection of Soybean mosaic virus
Development of a lateral-flow assay (LFA) for rapid detection of Soybean mosaic virus
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开发用于快速检测大豆花叶病毒的侧向流动测定（LFA）
DOI：
10.1016/j.jviromet.2016.05.011
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发表时间：
2016-09-01
影响因子：
3.1
通讯作者：
Tao, Xiao-rong
中科院分区：
文献类型：
--
作者：
Zhu, Min;Zhang, Wen-na;Tao, Xiao-rong
大豆花叶病毒（SMV）是大豆中最常见的病毒，在世界上许多国家对作物生产和种质资源衰退构成严重威胁。在这项研究中，开发了一种高度实用和快速的横向流动检测方法（LFA）来检测SMV。原核表达并纯化了SMV外壳蛋白（CP），用于小鼠免疫。在杂交瘤细胞系生成后，选择4种抗smv单克隆抗体。然后使用双抗体夹心策略组装lfa条带。用组装好的LFA-strip检测smv感染的叶片样品时，5-10 min内检测株系出现阳性粉红色。该条带仅对SMV呈阳性，对其他病毒无阳性反应，可用于检测稀释度为800倍的感染叶片样品。LFA可用于检测侵染大豆叶片组织和种子中的SMV。据我们所知，这是第一份开发用于检测SMV的LFA的报告。本研究建立的实用、快速、特异的检测方法可广泛应用于实验室和现场的SMV诊断和监测。(C) 2016 Elsevier B.V.版权所有
Soybean mosaic virus (SMV) is the most common virus in soybean and poses a serious threat to crop production and germplasm recession in many countries worldwide. In this study, a highly practical and rapid lateral-flow assay (LFA) was developed for the detection of SMV. The SMV coat protein (CP) was prokaryotically expressed and purified to immunize mice. After generation of hybridoma cell lines, four anti-SMV monoclonal antibodies were selected. The LFA-strip was then assembled using a double-antibody sandwich strategy. When the SMV-infected leaf sample was assayed using the assembled LFA-strip, the positive pink color appeared in the test line within 5-10 min. The strip only gave positive results with SMV and not other viruses tested and could be used to detect 800 fold dilutions of infected leaf samples. The LFA could be used to detect SMV in infected leaf tissue as well as soybean seeds. To our knowledge, this is the first report of the development of a LFA for the detection of SMV. The practical, rapid and specific assay that was developed in this study can be widely applied to the diagnosis and surveillance of SMV in the laboratory and the field. (C) 2016 Elsevier B.V. All rights reserved.