STRUCTURAL ORGANIZATION OF MOUSE PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR-GAMMA (MPPAR-GAMMA) GENE - ALTERNATIVE PROMOTER USE AND DIFFERENT SPLICING YIELD 2 MPPAR-GAMMA ISOFORMS
STRUCTURAL ORGANIZATION OF MOUSE PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR-GAMMA (MPPAR-GAMMA) GENE - ALTERNATIVE PROMOTER USE AND DIFFERENT SPLICING YIELD 2 MPPAR-GAMMA ISOFORMS
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DOI：
10.1073/pnas.92.17.7921
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发表时间：
1995-08-15
影响因子：
11.1
通讯作者：
REDDY, JK
中科院分区：
文献类型：
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作者：
ZHU, YJ;KORENBERG, JR;REDDY, JK
为了深入了解过氧化物酶体增殖物激活受体(PPAR)亚型的表达调控，我们确定了小鼠PPARγ(MPPAR Gamma)基因的结构组织，该基因延伸>；MPPAR Gamma 2基因编码mPPAR Gamma 1第一个ATG密码子的N端30个氨基酸，并揭示了一个不同的5‘非翻译序列。我们发现mPPAR Gamma 1mRNA由8个外显子编码，而mPPAR Gamma 2 mRNA由7个外显子编码，mPPAR Gamma 1 mRNA的5’未翻译序列大部分由2个外显子编码，而mPPAR Gamma 2的5‘端未翻译序列和额外的30个氨基酸由1个外显子编码它位于编码mPPAR Gamma 1的第二和第三外显子之间，mPPAR Gamma基因的最后6个外显子编码mPPAR Gamma 1和mPPAR Gamma 2亚型的相同序列，mPPAR Gamma 1和mPPAR Gamma 2亚型由不同的启动子转录而来，mPPAR Gamma基因通过生物素标记的探针原位杂交被定位在6号染色体E3-F1上。这些结果证明，至少有一个PPAR基因产生不止一种蛋白质产物，类似于维甲酸X受体和维甲酸受体基因。不同启动子转录的多个PPAR亚型的存在可以增加配体和组织特异性转录反应的多样性。
To gain insight into the regulation of expression of peroxisome proliferator-activated receptor (PPAR) isoforms, we have determined the structural organization of the mouse PPAR gamma (mPPAR gamma) gene, This gene extends >105 kb and gives rise to two mRNAs (mPPAR gamma 1 and mPPAR gamma 2) that differ at their 5' ends, The mPPAR gamma 2 cDNA encodes an additional 30 amino acids N-terminal to the first ATG codon of mPPAR gamma 1 and reveals a different 5' untranslated sequence, We show that mPPAR gamma 1 mRNA is encoded by eight exons, whereas the mPPAR gamma 2 mRNA is encoded by seven exons, Most of the 5' untranslated sequence of mPPAR gamma 1 mRNA is encoded by two exons, whereas the 5' untranslated sequence and the extra 30 N-terminal amino acids of mPPAR gamma 2 are encoded by one exon, which is located between the second and third exons coding for mPPAR gamma 1, The last six exons of mPPAR gamma gene code for identical sequences in mPPAR gamma 1 and mPPAR gamma 2 isoforms, The mPPAR gamma 1 and mPPAR gamma 2 isoforms are transcribed from different promoters, The mPPAR gamma gene has been mapped to chromosome 6 E3-F1 by in situ hybridization using a biotin-labeled probe. These results establish that at least one of the PPAR genes yields more than one protein product, similar to that encountered with retinoid X receptor and retinoic acid receptor genes. The existence of multiple PPAR isoforms transcribed from different promoters could increase the diversity of ligand and tissue-specific transcriptional responses.