Histochemical and electron microscope investigations on medullary bone
Histochemical and electron microscope investigations on medullary bone
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髓质骨的组织化学和电子显微镜研究
DOI：
10.1007/bf00218592
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发表时间：
1975
影响因子：
3.6
通讯作者：
G. Gherardi
中科院分区：
文献类型：
--
作者：
E. Bonucci;G. Gherardi
给鸽子服用卵泡素可以刺激股骨和其他长骨骨髓腔中的骨髓骨发育。它是一种特殊的骨组织，不参与机械功能，在蛋壳形成之前迅速被重吸收。髓骨的形成和重吸收与其他类型的骨相同。因此，由于其非常快的形成和吸收速率，它是研究成骨细胞、骨细胞和骨细胞活性以及钙化过程的理想组织。骨髓被PAS、阿尔新蓝和胶体铁深染，甲苯胺蓝染色后呈异染性。这表明它的基质基质含有相对高含量的糖蛋白和酸性蛋白聚糖。钙化最初发生在基质囊泡（或钙化球）中，这些囊泡在类骨组织的胶原纤维之间非常多，并逐渐扩散到周围的纤维间基质中。这些发现证实，在髓骨中，如同在其他类型的钙化组织中一样，无机物质最初位于钙化球内，然后依次与有机的、非胶原的、丝状有机结构（晶体幽灵），可能代表钙盐沉积的框架。
SummaryFolliculin administration to pigeons stimulates the development of medullary bone in marrow spaces of the femora and other long bones. It is a specialized osseus tissue not devoted to mechanical functions and which is rapidly reabsorbed before egg-shell formation.Medullary bone is formed and reabsorbed in the same way as other types of bone. Consequently, because of its very rapid rate of formation and resorption, it represents an ideal tissue for studying osteoblastic, osteoclastic and osteocytic activity, and the calcification process.Medullary bone is deeply stained by PAS, Alcian blue and colloidal iron and is metachromatic after toluidine blue staining. This shows that its interfibrillary ground substance contains relatively high amounts of glycoproteins and acid proteoglycans.Calcification initially occurs in matrix vesicles (or calcifying globules) which are very numerous between the collagen fibrils of the osteoid tissue, and successively spreads into the surrounding interfibrillar matrix. Here, the crystals are closely related to thin, filament-like organic structures which seem to be components of ground substance proteoglycans.These findings confirm that in medullary bone, as in other types of calcifying tissue, the inorganic substance is initially laid down within calcifying globules and is successively closely related to organic, non-collagenous, filamentous organic structures (crystal ghosts) which probably represent a framework for calcium salt deposition.