Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells
Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells
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DOI：
10.1016/s0006-291x(03)01245-2
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发表时间：
2003-08-01
影响因子：
3.1
通讯作者：
Kitamura, T
中科院分区：
文献类型：
--
作者：
Kumagai, H;Oki, T;Kitamura, T
我们已经在小鼠抗原刺激的骨髓来源的肥大细胞cDNA文库中鉴定了两个小鼠的cDNA。这两种基因都编码I型跨膜蛋白。这些基因紧密定位于小鼠染色体I I的远端区域，不仅在肥大细胞中表达，而且在白细胞中也广泛表达。其编码蛋白的胞外区含有单个可变免疫球蛋白(Ig)基序，与氨基酸的同源性约为90%，表明它们由一对分子组成，属于Ig超家族。我们将这些分子命名为白细胞单Ig样受体1和2(LMIR1和2)。LMIR1的胞内区含有几个基于酪氨酸的免疫受体抑制基序(ITIMs)。当交联时，胞内结构域被酪氨酸磷酸化，并能够募集酪氨酸磷酸酶，SHP-1和SHP-2，以及肌醇多聚磷酸5-磷酸酶，SHIP。另一方面，LMIR2含有一个短的细胞质尾巴和一个特征的跨膜结构域，该结构域携带两个带正电的氨基酸，与三种免疫受体酪氨酸激活基序(ITAM)分子DAP10、DAP12和FcRGamma相关。这些发现表明，一对新的ITIM/ITAM受体LMIR1和LMIR2通过尚未确定的配体(S)调节肥大细胞介导的炎症反应。(C)2003 Elsevier Inc.保留所有权利。
We have identified and characterized two mouse cDNAs in a mouse antigen-stimulated bone marrow-derived mast cell cDNA library. both of which encode type I transmembrane proteins. The genes were closely mapped in the distal region of mouse chromosome I I and expressed not only in mast cells but also widely in leukocytes. The extracellular domains of their encoded proteins contain a single variable immunoglobulin (Ig) motif sharing about 90% identity with amino acids, showing that they comprise a pair of molecules and belong to the Ig superfamily. We named these molecules leukocyte mono-Ig-like receptor1 and 2 (LMIR1 and 2). The intracellular domain of LMIR1 contains several immunoreceptor tyrosine-based inhibition motifs (ITIMs). When cross-linked, the intracellular domain was tyrosine phosphorylated and capable of recruiting tyrosine phosphatases, SHP-1 and SHP-2 and inositol polyphosphate 5-phosphatase, SHIP. LMIR2, on the other hand, contains a short cytoplasmic tail and a characteristic transmembrane domain carrying two positively charged amino acids associated with three kinds of immunoreceptor tyrosine-based activation motif (ITAM)-bearing molecules, DAP10, DAP12, and FcRgamma. These findings suggest that a new pair of ITIM/ITAM -bearing receptors, LMIR1 and 2, regulate mast cell-mediated inflammatory responses through yet to be defined ligand(s). (C) 2003 Elsevier Inc. All rights reserved.