Resistance Status of Busseola fusca (Lepidoptera: Noctuidae) Populations to Single- and Stacked-Gene Bt Maize in South Africa.
Resistance Status of Busseola fusca (Lepidoptera: Noctuidae) Populations to Single- and Stacked-Gene Bt Maize in South Africa.
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南非 Busseola fusca（鳞翅目：夜蛾科）种群对单基因和叠加基因 Bt 玉米的抗性状况。
DOI：
10.1093/jee/toy306
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发表时间：
2019
影响因子：
2.2
通讯作者：
Strydom E
中科院分区：
文献类型：
--
作者：
Strydom E
自 1998 年起，表达 Cry 苏云金芽孢杆菌 δ-内毒素的转基因 Bt 玉米已在南非种植，用于控制 Busseola fusca。在整个玉米产区的许多地方，Busseola fusca 对 Cry1Ab Bt 玉米具有抗性。释放前评估（1994-1996）B的固有易感性和释放后评估（1998-2011）抗药性状态。 fusca 专注于有限数量的害虫种群。本研究报告了当前 10B 的敏感性水平。对 2013 年至 2017 年期间对 fusca 种群进行了评估，并将该数据与之前报道的该害虫在 Bt 玉米上的存活数据进行了比较，包括 1994 年和 1995 年期间进行的预释放评估数据。对表达 Cry1Ab 或 Cry1A.105+Cry2Ab2（堆叠事件）蛋白的玉米事件的植物组织进行了幼虫摄食生物测定，并记录了存活率和不同的生活史参数。结果显示 B 的易感性水平发生了变化。 fuscato Bt 玉米。单基因事件的预发布评估显示，Bt 玉米叶组织上的幼虫存活率非常低，而 10 年后的研究和当前的研究报告，Cry1Ab 玉米上的存活率分别高达 40% 和 100%。虽然没有幼虫在堆积事件中完成其生命周期，但本研究中较高的 LT50 值表明 B.B. 的易感性发生了变化。 fuscato 叠加基因事件，并强调基线信息和监测害虫种群对 Bt 玉米易感性的重要性。
Transgenic Bt maize expressing Cry insecticidal δ-endotoxins ofBacillus thuringiensishas been cultivated in South Africa for the control ofBusseola fuscasince 1998.Busseola fuscais resistant to Cry1Ab Bt maize at many localities throughout the maize production region. Pre-release evaluation (1994–1996) of the inherent susceptibility and post-release assessments (1998–2011) of resistance status ofB. fuscafocused on a limited number of pest populations. This study reports the current levels of susceptibility of 10B. fuscapopulations evaluated between 2013 and 2017 and compared this data with previously reported data on the survival of this pest on Bt maize, including data of pre-release evaluations done during 1994 and 1995. Larval feeding bioassays in which plant tissue of maize events expressing either Cry1Ab or Cry1A.105+Cry2Ab2 (stacked event) proteins were conducted and survival and different life history parameters recorded. Results show a shift in levels of susceptibility ofB. fuscato Bt maize. Pre-release evaluation of the single-gene event showed very low larval survival on Bt maize leaf tissue while studies 10 yr later and the current study reported survival of up to 40% and 100% on Cry1Ab maize, respectively. While no larvae completed their life cycle on the stacked event, higher LT50 values in this study indicate a shift in susceptibility ofB. fuscato the stacked-gene event and highlight the importance of baseline information and monitoring of pest populations for their susceptibility to Bt maize.