Characterization of a wild rabies virus isolate of porcine origin in China
Characterization of a wild rabies virus isolate of porcine origin in China
复制标题
中国猪源野生狂犬病病毒分离株的鉴定。
DOI：
10.1016/j.meegid.2013.03.046
复制
发表时间：
2013-07-01
影响因子：
3.2
通讯作者：
Guo, Xiaofeng
中科院分区：
文献类型：
--
作者：
Luo, Yongwen;Zhang, Ying;Guo, Xiaofeng
狂犬病病毒（RABV）在世界范围内的各种哺乳动物中传播，可引起致命的脑脊髓炎。GD-SH-01是从中国狂犬病猪中分离到的一种街头狂犬病病毒。我们研究了GD-SH-01在乳鼠和成年小鼠中的致病性，并比较了培养物中NA和BHK-21细胞对GD-SH-01和CVS-24感染的易感性。确定了完整的GD-SH-01基因组序列，并与已知的RABV野生株进行了比较，以了解允许RABV在新宿主（如猪）中传播和适应的突变和遗传多样性。结果表明，GD-SH-01具有华南地区强毒株的特征，尽管在小鼠脑内的复制水平较低，但其致病指数仍高于CVS-24。在基因组中发现了多达47个独特的核苷酸替换，包括5个错义突变。这些数据为进一步了解RABV在不同宿主中的传播机制和遗传变异提供了有用的信息。(C)2013爱思唯尔有限公司版权所有。
Rabies virus (RABV) that circulates worldwide in a variety of mammals can cause fatal encephalomyelitis. GD-SH-01, a street rabies virus, was isolated from a rabid pig in China. We investigated the pathogenicity of GD-SH-01 in suckling and adult mice, and compared the susceptibility of NA and BHK-21 cells in the culture to infection by GD-SH-01 and CVS-24. The complete GD-SH-01 genome sequence was determined and compared with known RABV wild strains to understand the mutations and genetic diversity that allow RABV to spread and adapt in new hosts, such as pigs. Our results suggest that GD-SH-01 possesses the characteristics of a virulent strain in Southern China and shows higher pathogenicity index than that of CVS-24 regardless of its lower level of replication in mouse brain. Up to 47 unique nucleotide substitutions were found in the genome, including five missense mutations. These data provide useful information for further understanding the transmission mechanism and the genetic variation of RABV in dissimilar hosts. (C) 2013 Elsevier B.V. All rights reserved.