A hand-powered microfluidic system for portable and low-waste sample discretization.
A hand-powered microfluidic system for portable and low-waste sample discretization.
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DOI：
10.1039/d1lc00448d
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发表时间：
2021-08
期刊：
影响因子：
6.1
通讯作者：
Tengbao Xie;Ping Wang;Lei Wu;Bangyong Sun;Q. Zhao;Gang Li
中科院分区：
文献类型：
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作者：
Tengbao Xie;Ping Wang;Lei Wu;Bangyong Sun;Q. Zhao;Gang Li
在这项工作中，我们提出了一个简单的和无设备的系统，离散样品成成千上万的离散量在几十秒。与传统的样品离散化系统，需要庞大的注射泵，压力控制器，或真空设备，我们的系统只需要一张水溶性薄膜，手动操作的注射器，和一个包含高密度微室阵列的微流体装置。在该系统中，水溶性膜密封装置入口以形成封闭的通道-腔室系统，而注射器用于在封闭系统中产生真空。得益于真空吸附产生的高负压和密封水溶性膜的溶解触发门控机制，加载到装置入口的含水样品可以快速地被分隔成成千上万个隔离的腔室，而不需要任何昂贵的泵送系统。我们证明了该系统的实用性，利用它的数字PCR。我们相信，这种简单的离散化系统将得到广泛的应用，例如在数字生物测定、单细胞分析和即时诊断中。
In this work, we present a simple and equipment-free system for discretizing samples into tens of thousands of discrete volumes in tens of seconds. Unlike conventional sample discretization systems that require bulky syringe pumps, pressure controllers, or vacuum equipment, our system requires only a sheet of water-soluble film, a hand-operated syringe, and a microfluidic device containing a high-density microchamber array. In this system, the water-soluble film seals the device inlet to form a closed channel-chamber system, while the syringe is used to create a vacuum in the closed system. Benefitting from the high negative pressure created by syringe-vacuum and the dissolution-triggered gating mechanism of the sealing water-soluble film, the aqueous sample loaded into the device inlet can be rapidly partitioned into tens of thousands of isolated chambers without the need for any expensive pumping systems. We demonstrated the utility of this system by exploiting it for digital PCR. We believe that this simple discretization system will find broad applications, such as in digital bioassays, single-cell analysis, and point-of-care diagnostics.