The efficacy of conditioned media of adipose-derived stem cells combined with ablative carbon dioxide fractional resurfacing for atrophic acne scars and skin rejuvenation
The efficacy of conditioned media of adipose-derived stem cells combined with ablative carbon dioxide fractional resurfacing for atrophic acne scars and skin rejuvenation
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脂肪干细胞条件培养基联合消融性二氧化碳局部换肤治疗萎缩性痤疮疤痕和皮肤再生的功效
DOI：
10.3109/14764172.2015.1114638
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发表时间：
2016-01-01
影响因子：
1.2
通讯作者：
Luo, Dan
中科院分区：
文献类型：
--
作者：
Zhou, Bing-rong;Zhang, Ting;Luo, Dan
目的：评价脂肪来源干细胞条件培养液(ADSC-CM)对面部萎缩性痤疮瘢痕患者或有皮肤嫩肤需求的患者进行部分二氧化碳激光表面重建术(FxCR)后的疗效和不良反应。材料和方法：22名受试者随机分为两组。皮肤嫩肤组9例，痤疮疤痕组13例，均接受了3次FxCR治疗。随机选取一侧面部FxCR部位进行ADSC-CM检查。在基线、第一次治疗后1周和每次治疗后1个月进行评估。结果评估包括主观满意度评分、临床盲法评估、粗糙度、弹性、皮肤水份、经皮水分损失(TEWL)、红斑和黑色素指数等生物物理参数。用苏木精-伊红、马森氏三色染色和戈莫里氏醛品红染色对皮肤嫩肤组的一例患者的活检组织进行分析。结果：在痤疮疤痕组和皮肤嫩肤组，ADSC-CM联合FxCR均提高了受试者的满意度、弹性、皮肤水份和皮肤弹性，降低了TEWL、粗糙度和黑色素指数。组织学分析显示，ADSC-CM使真皮胶原密度、弹性蛋白密度增加，且排列整齐。结论：ADSC-CM联合FxCR是治疗萎缩性痤疮瘢痕和皮肤嫩肤的良好综合疗法。试验注册：JSPH2012-082-2012年2月14日注册
Objective: To evaluate the effects of conditioned medium of adipose-derived stem cells (ADSC-CM) on efficacy and side effects after fractional carbon dioxide laser resurfacing (FxCR) when treating subjects with facial atrophic acne scars or with skin rejuvenation needs. Materials and methods: Twenty-two subjects were enrolled in the study and divided into two groups. Nine subjects were included in skin rejuvenation group and thirteen subjects were included in acne scar group, and all subjects underwent three sessions of FxCR. ADSC-CM was applied on FxCR site of one randomly selected face side. Evaluations were done at baseline, 1 week after first treatment, and 1 month after each treatment. The outcome assessments included subjective satisfaction scale; blinded clinical assessment; and the biophysical parameters of roughness, elasticity, skin hydration, transepidermal water loss (TEWL), and the erythema and melanin index. Biopsies taken from one subject in skin rejuvenation group were analyzed using hematoxylin and eosin, Masson's Trichrome, and Gomori's aldehyde fuchsin staining. Results: ADSC-CM combined with FxCR increased subject satisfaction, elasticity, skin hydration, and skin elasticity and decreased TEWL, roughness, and the melanin index in both acne scars and skin rejuvenation groups. Histologic analysis showed that ADSC-CM increased dermal collagen density, elastin density, and arranged them in order. Conclusion: ADSC-CM with FxCR is a good combination therapy for treating atrophic acne scars and skin rejuvenation. Trial registration: JSPH2012-082 – Registered 14 Feb 2012