Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons
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DOI：
10.7554/elife.39786
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发表时间：
2018-10-08
期刊：
影响因子：
7.7
通讯作者：
Rayport, Stephen
中科院分区：
文献类型：
--
作者：
Chuhma, Nao;Mingote, Susana;Rayport, Stephen
多巴胺神经元在腹侧和背侧纹状体（dStr）有不同的突触活动，但这种异质性是否延伸到dStr亚区尚未得到解决。我们已经发现，小鼠脑切片中dStr多巴胺神经元末梢的光遗传学激活暂停了内侧和外侧亚区中胆碱能中间神经元的放电，而在外侧亚区中，由于随后的激发，暂停时间较短。这种兴奋主要由代谢型谷氨酸受体1（mGluR1）介导，部分由与瞬时受体电位通道3和7偶联的多巴胺D1样受体介导。DA神经元不向内侧dStr的多刺投射神经元发出信号，而它们在外侧dStr引起离子型谷氨酸反应。介导这些兴奋性信号的DA神经元位于黑质（substantia nigra，SN）。因此，SN多巴胺神经元在不同的dStr亚区的不同突触后神经元中参与不同的受体，以传达明显不同的信号。
Dopamine neurons have different synaptic actions in the ventral and dorsal striatum (dStr), but whether this heterogeneity extends to dStr subregions has not been addressed. We have found that optogenetic activation of dStr dopamine neuron terminals in mouse brain slices pauses the firing of cholinergic interneurons in both the medial and lateral subregions, while in the lateral subregion the pause is shorter due to a subsequent excitation. This excitation is mediated mainly by metabotropic glutamate receptor 1 (mGluR1) and partially by dopamine D1-like receptors coupled to transient receptor potential channel 3 and 7. DA neurons do not signal to spiny projection neurons in the medial dStr, while they elicit ionotropic glutamate responses in the lateral dStr. The DA neurons mediating these excitatory signals are in the substantia nigra (SN). Thus, SN dopamine neurons engage different receptors in different postsynaptic neurons in different dStr subregions to convey strikingly different signals.