Characteristics of the genome of Arsenophonus nasoniae, son-killer bacterium of the wasp Nasonia
Characteristics of the genome of Arsenophonus nasoniae, son-killer bacterium of the wasp Nasonia
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DOI：
10.1111/j.1365-2583.2009.00950.x
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发表时间：
2010-02-01
影响因子：
2.6
通讯作者：
Colbourne, J. K.
中科院分区：
文献类型：
--
作者：
Darby, A. C.;Choi, J. -H.;Colbourne, J. K.
本文报道了玻璃假单胞菌的杀虫细菌arcophonus nasoniae的基因组草图序列的性质。这项研究的基因组序列数据是第一个杀死男性的细菌，与其他测序的共生体相比，它代表了一种不寻常的微生物，它有常规的垂直和水平传播，两个交替的宿主，并且可以在无细胞的培养基上培养。得到的序列总计约3.5Mbp，并被注释为包含3332个预测的开放阅读框架(ORF)。因此，对于昆虫共生体来说，亚瑟氏菌代表了一个相对较大的基因组。注释的ORF集合表明，该微生物能够发挥广泛的代谢功能，远远超出迄今已测序的生殖寄生虫基因组的范围，更类似于水平传播的和次生共生体。我们还发现了沃尔巴克氏共生体的基因转移，以及与其他伽马蛋白细菌共生体的噬菌体交换的证据。这些发现反映了一种细菌的复杂生物学，这种细菌能够在抵抗免疫反应的同时生活、入侵和生存在多个宿主环境中。
We report the properties of a draft genome sequence of the bacterium Arsenophonus nasoniae, sonkiller bacterium of Nasonia vitripennis. The genome sequence data from this study are the first for a male-killing bacterium, and represent a microorganism that is unusual compared with other sequenced symbionts, in having routine vertical and horizontal transmission, two alternating hosts, and being culturable on cell-free media. The resulting sequence totals c. 3.5 Mbp and is annotated to contain 3332 predicted open reading frames (ORFs). Therefore, Arsenophonus represents a relatively large genome for an insect symbiont. The annotated ORF set suggests that the microbe is capable of a broad array of metabolic functions, well beyond those found for reproductive parasite genomes sequenced to date and more akin to horizontally transmitted and secondary symbionts. We also find evidence of genetic transfer from Wolbachia symbionts, and phage exchange with other gammaproteobacterial symbionts. These findings reflect the complex biology of a bacterium that is able to live, invade and survive multiple host environments while resisting immune responses.