2n GAMETES IN DIPLOID SOLANUM: FREQUENCY AND TYPES OF SPINDLE ABNORMALITIES
2n GAMETES IN DIPLOID SOLANUM: FREQUENCY AND TYPES OF SPINDLE ABNORMALITIES
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DOI：
10.1139/g82-032
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发表时间：
1982-06
期刊：
影响因子：
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通讯作者：
R. Veilleux;N. McHale;F. Lauer
中科院分区：
文献类型：
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作者：
R. Veilleux;N. McHale;F. Lauer
对二倍体（2x = 24）茄Solanum phureja Juz的4个无性系进行了细胞学检查，结果表明，在2n花粉中存在细胞学异常。和布克。两个品种（148-17和127-14）的2n花粉产量可变，两个品种（154-1和B2-11）的2n花粉产量一直很高。在第二次减数分裂中观察到三种类型的异常纺锤体方向：融合，三极和平行纺锤体（分别为FS，TPS和PS）。所有四种基因型都有fs。3个有tps，ps最少，仅发生在两种基因型中。芽的横截面显示，所有三个异常可能发生在相邻的细胞室。在所有的基因型，FS占主导地位，在高水平的表达2n配子。然而，在较低的表达水平下（即低于60%，仅发生在148-17和127-14中），tps和ps的频率增加。一般来说，很少有证据表明，二分体（由fs或ps产生）或四分体（由正常分裂产生）在室的横截面内的定位，但所有四分体的52.4%是在室的横截面内。
Cytological disturbances resulting in 2n pollen were examined in four clones of diploid (2x = 24) Solanum phureja Juz. and Buk.; two (148-17 and 127-14) were variable and two (154-1 and B2-11) were consistently high in 2n pollen production. Three types of abnormal spindle orientation were observed at the second meiotic division: fused, tripolar, and parallel spindles (fs, tps, and ps, respectively). All four genotypes had fs. Three had tps, and ps was least frequent, occuring in two genotypes only. Cross-sections of buds revealed that all three abnormalities can occur in adjacent cells of a locule. In all genotypes, fs predominated at high levels of expression of 2n gametes. At lower levels of expression (i.e. less than 60%, which occurred only in 148-17 and 127-14), however, the frequencies of tps and ps increased. In general, there was little evidence for localization of dyads (resulting from fs or ps) or tetrads (resulting from normal divisions) within cross sections of locules, but 52.4% of all tetrad...