PNMT-containing catecholaminergic neurons are not necessarily adrenergic.
PNMT-containing catecholaminergic neurons are not necessarily adrenergic.
复制标题
含有 PNMT 的儿茶酚胺能神经元不一定是肾上腺素能神经元。
DOI：
10.1016/0006-8993(89)90490-3
复制
发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Sved,AF
中科院分区：
文献类型：
--
作者：
Sved,AF
脊髓中的中间外侧细胞柱（IML）由延髓腹外侧（C1细胞群）的含苯乙醇胺-N-甲基转移酶（PNMT）的神经元密集支配。本研究使用了一种灵敏的HPLC-EC测定儿茶酚胺定量肾上腺素水平的区域的脊髓含有IML。在富含IML的胸髓以及整个胸髓的解剖中，肾上腺素水平低于测定的灵敏度限度，表明肾上腺素水平低于0.2 pg/mg组织。在IML区域，肾上腺素水平低于去甲肾上腺素含量的0.05%。因此，即使IML含有密集的神经末梢网络，其对PNMT和肾上腺素生物合成所需的其他酶进行化学染色，也不能在IML中检测到肾上腺素。这些结果表明，含有肾上腺素生物合成所需的所有酶的神经末梢不一定是肾上腺素能的。
The intermediolateral cell column (IML) in the spinal cord is densely innervated by the phenylethanolamine-N-methyltransferase (PNMT)-containing neurons of the ventrolateral medulla (C1 cell group). The present study used a sensitive HPLC-EC assay for catecholamines to quantitate epinephrine levels in the region of the spinal cord containing the IML. In dissections of thoracic cord enriched in IML, as well as total thoracic cord, epinephrine levels were below the limit of sensitivity of the assay, indicating that epinephrine levels were less than 0.2 pg/mg tissue. In the region of the IML, epinephrine levels were less than 0.05% of the norepinephrine content. Thus, no epinephrine could be detected in the IML even though the IML contains a dense network of nerve terminals that stain immunohistochemically for PNMT and the other enzymes required for epinephrine biosynthesis. These results suggest that nerve terminals that contain all the enzymes required for epinephrine biosynthesis are not necessarily adrenergic.