WALL ADSORPTION OF SMALL ANIONS IN CAPILLARY ZONE ELECTROPHORESIS INDUCED BY CATIONIC TRACE CONSTITUENTS OF THE BUFFER
WALL ADSORPTION OF SMALL ANIONS IN CAPILLARY ZONE ELECTROPHORESIS INDUCED BY CATIONIC TRACE CONSTITUENTS OF THE BUFFER
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DOI：
10.1016/0021-9673(94)80048-0
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发表时间：
1994-09-30
影响因子：
4.1
通讯作者：
KENNDLER, E
中科院分区：
文献类型：
--
作者：
GASSNER, B;FRIEDL, W;KENNDLER, E
Fe（III）作为一种微量成分存在于缓冲液中，即使在ppb浓度范围内，也会在涂覆和未涂覆的熔融二氧化硅毛细管中诱导小阴离子的壁吸附。对于二硝基苯甲酸酯和三硝基苯甲酸酯，羧酸基处于邻位时，吸附效果明显，导致尾峰严重，迁移次数的重现性较低。与邻位衍生物相比，1,3-或1,4位羧酸基苯甲酸酯受影响较小。阴离子的吸附与附着在毛细管壁上的Fe（III）有关。
Fe(III), present as a trace constituent in the buffer, induces wall adsorption of small anions in coated and uncoated fused-silica capillaries even in the ppb concentration range. For di- and tribasic benzoates, drastic adsorption effects were found when the carboxylate groups were in an ortho position, leading to severely tailing peaks and a lower reproducibility of migration times. Benzoates with carboxylate groups in 1,3- or 1,4-positions were less affected than the ortho derivatives. The adsorption of the anions is related to Fe(III) attached to the capillary wall.