JUNIN VIRUS-INFECTION OF GUINEA-PIGS - IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES OF HEMATOPOIETIC-TISSUE
JUNIN VIRUS-INFECTION OF GUINEA-PIGS - IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES OF HEMATOPOIETIC-TISSUE
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DOI：
10.1093/infdis/143.1.7
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发表时间：
1981-01-01
影响因子：
6.4
通讯作者：
ARANA, RM
中科院分区：
文献类型：
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作者：
CARBALLAL, G;COSSIO, PM;ARANA, RM
病毒抗原、细胞病变效应(CPE)和血液和淋巴组织中病毒滴度之间的关系表明，Junin病毒对感染XJ原型毒株103LD50的豚鼠的淋巴器官有直接的CPE。感染7天后，所有淋巴网状器官的感染性滴度均高于血液。病毒感染后第5天从骨髓和淋巴结中恢复，第10天在骨髓、脾和淋巴结中达到高峰，第7天在网状单核细胞胞浆中检测到颗粒状特异性荧光，巨核细胞呈阳性荧光，其他淋巴样细胞未见特异性染色。第9天可见骨髓、淋巴结和脾组织坏死。CPE主要表现为网织单核细胞和成髓细胞网状内质池过度发育。观察到典型的Junin病毒颗粒。网状细胞逐渐破坏，周围淋巴样细胞同时坏死。
An association between viral antigens, cytopathic effect (CPE), and viral titers in blood and lymphoid tissues suggests a direct CPE of Junin virus on the lymphpoietic organs of guinea pigs infected with 103 LD50 of the XJ prototype strain. After 7 days of infection, all lymphoreticular organs had infectivity titers higher than those for blood. Virus was recovered from bone marrow and lymph nodes at day 5 after infection; peak titers were obtained from bone marrow, spleen and lymph nodes after day 10. Granular specific fluorescence was detected in the cytoplasm of reticular monocytes after day 7; megakaryocytes showed positive fluorescence, but specific staining of other lymphoid cells was not observed. Necrosis of bone marrow, lymph nodes and spleen was observed after day 9. CPE consisted of overdevelopment of reticuloendoplasmic cisternae of reticulomonocytes and myeloblasts. Typical Junin virus particles were observed. Reticular cells were gradually destroyed, and simultaneous necrosis of surrounding lymphoid cells was observed.