A GENETIC APPROACH TO THE BIOGENESIS OF PEROXISOMES IN THE YEAST SACCHAROMYCES-CEREVISIAE
A GENETIC APPROACH TO THE BIOGENESIS OF PEROXISOMES IN THE YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/cbf.290100306
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发表时间：
1992-09-01
影响因子：
3.6
通讯作者：
KUNAU, WH
中科院分区：
文献类型：
--
作者：
ERDMANN, R;KUNAU, WH
芽殖酵母 Succhurom, vces cerevisiue 具有结合遗传、分子遗传和生化方法来研究细胞生物过程的优势。酿酒酵母突变体是剖析线粒体等亚细胞结构生物发生的有力工具。” 2 内质网，~.~ g~ lgi，~ 液泡，6 以及最近的过氧化物酶体。~-'~酿酒酵母中过氧化物酶体的存在首先由 Avers 和 Federman 证明，''但与其他酵母和丝状真菌相比，'2.''酿酒酵母的过氧化物酶体尚未得到详细表征。这种信息的缺乏可以通过以下观察来解释：在最初选择的生长条件下，酿酒酵母中的过氧化物酶体非常罕见并且不存在。在一些酵母中，这种细胞器的增殖可以通过在不同的碳源和氮源上生长来诱导，但在酿酒酵母中诱导过氧化物酶体增殖的尝试长期以来都失败了。直到 Veenhuis 等人'" 和 Kunau 等人' 证明了过氧化物酶体 P 氧化的诱导性以及通过以油酸作为单一碳源生长在酵母中的过氧化物酶体增殖，在 S. ccw~ ci. siur~ 中也发现了大量的过氧化物酶体。'4.1 这一发现为开发分离过氧化物酶体1突变体的遗传筛选开辟了道路。 S.~ erevisiue.~ 在本报告中，我们回顾了通过结合遗传和生化方法来表征酿酒酵母中过氧化物酶体生物发生的首次尝试，我们描述了不同类型的过氧化物酶体突变体以及我们目前对主要突变体的了解。
The budding yeast Succhurom, vces cerevisiue offers the advantages of combined genetic, molecular genetic and biochemical approaches to study cell biological processes. S. cerevisiae mutants have been powerful tools to dissect the biogenesis of subcellular structures, such as mitochondria,'. 2 endoplasmic reti~ ulum,~.~ g~ lgi,~ vacuoles, 6 and most recently peroxi~ omes.~-'~The presence of peroxisomes in S. cerevisiue was first demonstrated by Avers and Federman,''but in contrast to other yeasts and filamentous fungi,'2.''peroxisomes of S. cerevisiue have not been characterized in great detail. This lack of information is explained by the observation that under the growth conditions originally chosen, peroxisomes in S. cerevisiue are very rare and~ rna1l. l~ In several yeasts, proliferation of this organelle can be induced by growth on distinct carbon and nitrogen sources1'but attempts to induce the proliferation of peroxisomes in S. cerevisiue failed for a long time. It was not until Veenhuis et d.'" and Kunau et al.'demonstrated the inducibi-lity of peroxisomal P-oxidation as well as peroxisome proliferation in yeasts by growth on oleic acid as the single carbon source that remarkable amounts of peroxisomes were also found in S. ccw~ ci. siur~.'4.1 This finding opened the way for the development of a genetic screen to isolate peroxisoma1 mutants of S.~ erevisiue.~ In this report we review the first attempts to characterize peroxisome biogenesis in S. cerevisiue by a combined genetic and biochemical approach. We describe the different types of peroxisomal mutants and our current knowledge of the primary