Minimum number of islets required to maintain euglycemia and their reduced immunogenicity after transplantation into diabetic mice.
Minimum number of islets required to maintain euglycemia and their reduced immunogenicity after transplantation into diabetic mice.
复制标题
维持血糖正常所需的最小胰岛数量及其在移植到糖尿病小鼠中后免疫原性降低。
DOI：
10.1097/00007890-199308000-00003
复制
发表时间：
1993
期刊：
影响因子：
6.2
通讯作者：
Maki,T
中科院分区：
文献类型：
--
作者：
Ohzato,H;Porter,J;Monaco,AP;Montana,E;Maki,T
In streptozocin (SZ)-induced diabetic mice, 200 islets, but not 50 islets, consistently restore euglycemia within 1 week of transplantation. To determine the minimum number of islets sufficient to maintain euglycemia in a diabetic mouse, we first transplanted 50 and 150 syngeneic islets simultaneously into the right (RK) and left kidney (LK), respectively, and then removed the LK 1 week post-transplantation. The remaining 50 islets maintained euglycemia in 8 of 11 mice with normal intravenous glucose tolerance tests (IVGTT). Protection of 50 islets for at least 7 days was necessary because removal of the 150 islets at 5 or 3 days resulted in a much lower incidence of persistent euglycemia. Similarly, 25 islets were capable of maintaining euglycemia in 2 of 9 mice once hyperglycemia was reversed by split-transplantation of 25 (RK) and 175 (LK) islets. To examine if 50-islet allografts survive longer than 200-islet allografts, we split-transplanted 50 DBA/2 islets in the RK and 150 islets of either B6 (syngeneic), DBA/2 (allogeneic), or C3H/He (third party allogeneic) mouse origin in the LK in 3 groups of diabetic C57BL/6 (B6) mice. The survival of 50 DBA/2 islets in each group after removal of the LK on day 7 was compared to that of 200 DBA/2 islets in control B6 mice. Maximum prolongation of allograft survival was obtained with 50 DBA/2 islets that were split-transplanted with syngeneic B6 islets. These results clearly demonstrate that 50 islets are sufficient to maintain normal glucose tolerance once euglycemia is induced by transplantation of a larger number (ie, 200) of islets and that 50 islet allografts are much less immunogenic than 200-islet allografts.
