CONCUSSIVE BRAIN INJURY IS ASSOCIATED WITH A PROLONGED ACCUMULATION OF CALCIUM - A CA-45 AUTORADIOGRAPHIC STUDY
CONCUSSIVE BRAIN INJURY IS ASSOCIATED WITH A PROLONGED ACCUMULATION OF CALCIUM - A CA-45 AUTORADIOGRAPHIC STUDY
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DOI：
10.1016/0006-8993(93)90064-t
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发表时间：
1993-10-08
期刊：
影响因子：
2.9
通讯作者：
BECKER, DP
中科院分区：
文献类型：
--
作者：
FINEMAN, I;HOVDA, DA;BECKER, DP
为了确定钙（Ca 2+）流量的程度和持续时间后，在大鼠的侧向液压冲击脑损伤，Ca-45放射自显影术用于研究动物立即，6，24和96小时后的侮辱。此外，进行细胞悬浮液研究以确定Ca-45的细胞通量的程度。利用光密度和/或闪烁计数来提供20种不同结构内Ca-45积累的相对测量。结果表明，在没有表现出大体形态学损伤的动物中，损伤后的Ca-45积累主要表现在同侧大脑皮质、背侧海马和纹状体内。这种积累持续了几天，到损伤后第4天恢复到对照水平。在持续形态学损伤的动物中，挫伤部位表现出Ca-45的显著积聚，其在4天的过程中没有自发地消退。我们得出结论，从这项工作中，Ca 2+流量是这个实验模型的创伤性损伤的主要组成部分。此外，根据细胞损伤的程度，Ca 2+的积累存在区域差异。最后，即使在本身不产生大体形态组织损伤的损伤中，Ca 2+的积累也可以持续至少48小时。
In order to determine the extent and duration of calcium (Ca2+) flux following a lateral fluid percussion brain injury in the rat, Ca-45 autoradiography was used to study animals immediately, 6, 24 and 96 h after the insult. In addition, cell suspension studies were conducted to determine the extent of cellular flux of Ca-45. Optical density and/or scintillation counting was utilized to provide a relative measure of Ca-45 accumulation within 20 different structures. The results indicated that in animals who exhibited no gross morphological damage, Ca-45 accumulation following injury was exhibited primarily within the ipsilateral cerebral cortex, dorsal hippocampus and striatum. This accumulation continued for several days returning to control levels by the 4th day after injury. In animals who sustained morphological damage, the contusion site exhibited a marked accumulation of Ca-45 which did not resolve spontaneously over the course of 4 days. We conclude from this work that Ca2+ flux is a major component of this experimental model of traumatic injury. Furthermore, that depending on the extent of cell damage, the accumulation of Ca2+ is regionally different. Finally, that even in an injury which by itself does not produce gross morphological tissue damage, accumulation of Ca2+ can continue for at least 48 h.