Cell-Free Supernatants from Probiotic Lactobacillus casei and Lactobacillus rhamnosus GG Decrease Colon Cancer Cell Invasion In Vitro
Cell-Free Supernatants from Probiotic Lactobacillus casei and Lactobacillus rhamnosus GG Decrease Colon Cancer Cell Invasion In Vitro
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DOI：
10.1080/01635581.2012.700758
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Maitin, Vatsala
中科院分区：
文献类型：
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作者：
Escamilla, Juanita;Lane, Michelle A.;Maitin, Vatsala
益生菌已被证明在结直肠癌发生中具有预防作用，但关于其在结直肠癌晚期，特别是转移中的预防潜力的研究有限。本研究探索了来自2种益生乳杆菌属的无细胞上清液（CFS）的潜力，干酪乳杆菌和鼠李糖乳杆菌GG，通过影响培养的转移性人结肠直肠癌细胞中基质金属蛋白酶-9（MMP-9）活性和紧密连接蛋白紧密连接蛋白-I（ZO-1）水平来抑制结肠癌细胞侵袭。用L.干酪湖（L. casei）rhamnosus或多形拟杆菌（Bacteroides thetaiotaomicron）（肠道细菌）;或用未接种的细菌生长培养基。用来自两种乳杆菌属的CFS处理降低结肠直肠细胞侵袭，但用来自B的CFS处理。但泰奥塔奥米克隆却没有。来自两种乳杆菌的CFS降低MMP-9并增加ZO-1蛋白水平。L.鼠李糖CFS也降低MMP-9活性。为了开始阐明传递这些应答的分泌的细菌因子，将乳杆菌属CFS分级成限定的分子量范围并评估细胞侵袭。分级显示抑制活性主要包含在>100 kDa和50-100 kDa级分中，表明抑制化合物可能是大分子，如蛋白质、核酸或多糖。
Probiotics have been shown to have a preventative role in colorectal carcinogenesis but research concerning their prophylactic potential in the later stages of colorectal cancer, specifically metastasis is limited. This study explored the potential of cell-free supernatants (CFS) from 2 probiotic Lactobacillus sp., Lactobacillus casei and Lactobacillus rhamnosus GG, to inhibit colon cancer cell invasion by influencing matrix metalloproteinase-9 (MMP-9) activity and levels of the tight junction protein zona occludens-1 (ZO-1) in cultured metastatic human colorectal carcinoma cells. HCT-116 cells were treated with CFS from L. casei, L. rhamnosus, or Bacteroides thetaiotaomicron (a gut commensal); or with uninoculated bacterial growth media. Treatment with CFS from both Lactobacillus sp. decreased colorectal cell invasion but treatment with CFS from B. thetaiotaomicron did not. CFS from both Lactobacillus sp. decreased MMP-9 and increased ZO-1 protein levels. L. rhamnosus CFS also lowered MMP-9 activity. To begin elucidating the secreted bacterial factor conveying these responses, Lactobacillus sp. CFS were fractionated into defined molecular weight ranges and cell invasion assessed. Fractionation revealed that the inhibitory activity was contained primarily in the >100 kDa and 50-100 kDa fractions, suggesting the inhibitory compound may be a macromolecule such as a protein, nucleic acid, or a polysaccharide.