Physiologically detectable bisphenol A impairs human sperm functions by reducing protein-tyrosine phosphorylation
Physiologically detectable bisphenol A impairs human sperm functions by reducing protein-tyrosine phosphorylation
复制标题
生理学上可检测到的双酚 A 通过降低蛋白质酪氨酸磷酸化来损害人类精子的功能。
DOI：
10.1016/j.ecoenv.2021.112418
复制
发表时间：
2021-06-17
影响因子：
6.8
通讯作者：
Zeng, Xu-hui
中科院分区：
文献类型：
--
作者：
Li, Na;Kang, Hang;Zeng, Xu-hui
背景资料：双酚A（BPA）是一种广泛使用的塑料单体和增塑剂，可在健康人的血液、尿液和精液中检测到，浓度范围为0.1 nM至10 nM。研究表明，体外暴露低至0.001 nM的BPA即可显着抑制小鼠精子活力和顶体反应。然而，目前还不清楚BPA在生理上可检测的浓度下是否会影响人类精子。研究方法：研究了不同浓度BPA（0、10-3、10-2、10-1、10、103 nM）对精子功能的影响，包括人精子活力、运动学参数、超活化和获能。结果如下：BPA可引起人精子活率、运动力和前向运动力显著下降，精子过度活化，获能和孕酮诱导的顶体反应。机制研究表明，BPA可抑制人精子蛋白酪氨酸磷酸化水平，但对精子钙信号无影响。结论：生理可检测浓度的BPA可能通过抑制人类精子蛋白酪氨酸磷酸化而损害人类精子功能，这意味着BPA的环境污染可能是导致男性不育的一个因素。
Background: Bisphenol A (BPA), a widely used plastic monomer and plasticizer, is detectable in blood, urine and semen of a healthy people, with concentrations ranging from 0.1 nM to 10 nM. It has been shown that in vitro exposure of BPA as low as 0.001 nM could significantly inhibited mouse sperm motility and acrosome reaction. However, it is still unclear whether BPA at those physiologically detectable concentration affects human sperm. Methods: The effects of different concentrations of BPA (0, 10-3, 10-2, 10-1, 10, 103 nM) on sperm functions were examined, including human sperm viability, kinematic parameters, hyperactivation and capacitation. Results: BPA caused a remarkable decline in human sperm viability, motility and progressive motility, hyperactivation, capacitation and progesterone-induced acrosome reaction. Mechanism studies showed that BPA could suppress the protein tyrosine phosphorylation level of human sperm, but had no effect on sperm calcium signaling. Conclusions: Physiologically detectable concentrations of BPA may impair human sperm functions via suppressing protein tyrosine phosphorylation of human sperm, implying that environmental pollution of BPA might be a factor contributing to male infertility.