Compatibility in the Biomphalaria glabrata/Echinostoma caproni model:: new candidate genes evidenced by a suppressive subtractive hybridization approach
Compatibility in the Biomphalaria glabrata/Echinostoma caproni model:: new candidate genes evidenced by a suppressive subtractive hybridization approach
复制标题
DOI：
10.1017/s0031182006001673
复制
发表时间：
2007-04-01
期刊：
影响因子：
2.4
通讯作者：
Mitta, G.
中科院分区：
文献类型：
--
作者：
Bouchut, A.;Coustau, C.;Mitta, G.
为了阐明蜗牛/棘口动物相容性的机制，进行了大量的分子研究，比较了对棘口动物caproni敏感或抗性的Bicanphalaria glabrata的转录本和蛋白质。这些研究集中在两个菌株的血浆和血细胞中，并揭示了一些转录本和/或蛋白质在菌株之间的差异表达。本研究的目的是通过构建消减文库，发展一种互补的转录组学方法。这项工作揭示了一些候选转录本已经确定在以前的研究（钙结合蛋白和糖酵解酶），以及新的候选转录本的差异，代表菌株之间的B。光滑的在这些新发现的基因中，我们发现了几个可能参与免疫过程的基因，编码蛋白酶，蛋白酶抑制剂，凝集素，花生素样分子和细胞粘附分子。
In order to elucidate mechanisms underlying snail/echinostome compatibility, numerous molecular studies comparing transcripts and proteins of Bicanphalaria glabrata susceptible or resistant to Echinostoma caproni were undertaken. These studies focused on plasma and haemocytes of the two strains and revealed that some transcripts and/or proteins were differentially expressed between strains. The aim of the present study,vas to develop a complementary transcriptomic approach by constructing subtractive libraries. This work revealed some candidate transcripts already identified in previous studies (calcium-binding proteins and glycolytic enzymes) as well as novel candidate transcripts that were differentially, represented between strains of B. glabrata. Among these newly identified genes, we revealed several genes potentially involved in immune processes encoding proteases, protease inhibitors, a lectin, an aplysianin-like molecule, and cell adhesion molecules.