The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
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DOI：
10.1038/nmat2013
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发表时间：
2007-12-01
期刊：
影响因子：
41.2
通讯作者：
Oreffo, Richard O. C.
中科院分区：
文献类型：
--
作者：
Dalby, Matthew J.;Gadegaard, Nikolaj;Oreffo, Richard O. C.
骨组织工程的一个关键原则是开发支架材料，该材料可以在没有化学处理的情况下刺激干细胞分化为成骨细胞，而不影响材料特性。目前，传统的植入材料由于被软组织封装而不是直接骨粘合而失效。在这里，我们展示了在没有成骨补充剂的情况下，使用纳米级紊乱刺激人类间充质干细胞 (MSC) 在体外产生骨矿物质。这种方法与用成骨培养基培养的细胞具有相似的效率。此外，目前的研究表明，与用成骨培养基处理的间充质干细胞相比，经过地形处理的间充质干细胞具有明显的分化特征，这对细胞治疗具有重要意义。
A key tenet of bone tissue engineering is the development of scaffold materials that can stimulate stem cell differentiation in the absence of chemical treatment to become osteoblasts without compromising material properties. At present, conventional implant materials fail owing to encapsulation by soft tissue, rather than direct bone bonding. Here, we demonstrate the use of nanoscale disorder to stimulate human mesenchymal stem cells ( MSCs) to produce bone mineral in vitro, in the absence of osteogenic supplements. This approach has similar efficiency to that of cells cultured with osteogenic media. In addition, the current studies show that topographically treated MSCs have a distinct differentiation profile compared with those treated with osteogenic media, which has implications for cell therapies.