EFFECTS OF CYTO-TOXIC MONOCLONAL-ANTIBODY SPECIFIC FOR T200 GLYCOPROTEIN ON FUNCTIONAL LYMPHOID-CELL POPULATIONS
EFFECTS OF CYTO-TOXIC MONOCLONAL-ANTIBODY SPECIFIC FOR T200 GLYCOPROTEIN ON FUNCTIONAL LYMPHOID-CELL POPULATIONS
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DOI：
10.1016/0008-8749(80)90335-4
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发表时间：
1980-01-01
影响因子：
4.3
通讯作者：
TROWBRIDGE, IS
中科院分区：
文献类型：
--
作者：
DENNERT, G;HYMAN, R;TROWBRIDGE, IS
抗T200 [鼠胸腺细胞]糖蛋白的单克隆抗体对胸腺细胞和成熟T淋巴细胞具有选择性细胞毒性。所有的T细胞功能测定，细胞介导的细胞毒性，辅助细胞活性和增殖的T细胞有丝分裂原或同种异体细胞，被取消了预先处理的脾细胞与抗T200抗体和补体。B细胞对脂多糖（LPS）和其他B细胞有丝分裂原的反应不受影响。虽然用抗T200抗体和补体处理脾细胞显著降低了它们对绵羊红细胞（SRBC）的体外抗体应答的能力，但通过加入SRBC引发的T辅助细胞可以恢复应答。用抗T200抗体和补体处理骨髓细胞并不能消除体内的集落形成单位-脾脏或胸腺细胞。显然，T淋巴细胞对补体介导的抗T200抗体裂解变得敏感，这是由于在它们进入胸腺之前不久或之后不久发生的细胞表面修饰。与Thy-1抗原相反，抗T200糖蛋白抗体的选择性杀伤不能通过细胞表面上T200糖蛋白表达的定量差异来容易地解释。活化细胞分析表明，T200糖蛋白表达在大多数胸腺细胞，脾脏和骨髓细胞相似的金额。
A monoclonal antibody against T200 [murine thymocyte] glycoprotein is selectively cytotoxic for thymocytes and mature T lymphocytes. All T-cell functions assayed, cell-mediated cytotoxicity, helper cell activity and proliferation in response to T-cell mitogens or allogeneic cells, were abolished by prior treatment of spleen cells with anti-T200 antibodies and complement. B cell responses to lipopolysaccharide (LPS) and other B-cell mitogens were unaffected. Although treatment of spleen cells with anti-T200 antibodies and complement markedly reduced their capacity to mount an in vitro antibody response to sheep red blood cells (SRBC), responsiveness could be restored by the addition of SRBC-primed T-helper cells. Treatment of bone marrow cells with anti-T200 antibodies and complement did not eliminate in vivo colony-forming units-spleen or prothymocytes. Apparently, T lymphocytes become sensitive to complement-mediated lysis by anti-T200 antibodies as a consequence of cell-surface modifications occurring shortly before or just after their entry into the thymus. In contrast to Thy-1 antigen, the selective killing by antibody against T200 glycoprotein cannot be readily accounted for by quantitative differences in the expression of T200 glycoprotein on the cell surface. Fluorescence-activated cell analysis showed that T200 glycoprotein was expressed in similar amounts on most thymocytes, spleen and bone marrow cells.