INHIBITION OF ENDOCYTOSIS BY ANTI-CLATHRIN ANTIBODIES
INHIBITION OF ENDOCYTOSIS BY ANTI-CLATHRIN ANTIBODIES
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DOI：
10.1016/0092-8674(87)90499-5
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发表时间：
1987-07-31
期刊：
影响因子：
64.5
通讯作者：
HELENIUS, A
中科院分区：
文献类型：
--
作者：
DOXSEY, SJ;BRODSKY, FM;HELENIUS, A
我们通过将单抗引入活细胞并确定它们对膜转运的影响，研究了胞浆蛋白，一种与包被的凹坑和小泡相关的细胞质蛋白的功能。用抗网状蛋白重链抗体后，网状蛋白聚集在细胞质中，质膜上被包被的凹坑减少，塞姆利基森林病毒的吸收内吞作用和液相内吞作用减少40%-50%。新合成的流感血凝素向质膜的转运没有变化。结果表明，CV-1细胞中的笼蛋白参与了液相摄取和受体介导的内吞作用，但不参与分泌途径中的结构性运输。
We examined the function of ciathrin, a cytoplasmic protein associated with coated pits and vesicles, by introducing monoclonal antibodies into living cells and determining their effects on membrane transport. When anti-clathrin heavy chain antibodies were used, the following effects were observed: clathrin became aggregated in the cytoplasm, the number of coated pits on the plasma membrane was reduced, and absorptive endocytosis of Semliki Forest virus and fluid-phase endocytosis were decreased by 40%-50%. No change in transport of newly synthesized influenza hemagglutinin to the plasma membrane was observed. The results indicated that clathrin in CV-1 cells is involved in fluid-phase uptake and receptor-mediated endocytosis, but not in constitutive transport within the secretory pathway.